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Maximizing ion transmission from atmospheric pressure into the vacuum of mass
spectrometers with a novel electrospray interface
#MMPMID25588722
Krutchinsky AN
; Padovan JC
; Cohen H
; Chait BT
J Am Soc Mass Spectrom
2015[Apr]; 26
(4
): 649-58
PMID25588722
show ga
We have discovered that an electrode containing a conical channel with a small
angular divergence can transmit into the vacuum almost 100% of an electrospray
ion current produced at atmospheric pressure. Our first implementation of such a
conical duct, which we term "ConDuct," uses a conductive plastic pipette tip
containing an approximately 1.6° divergent channel at its entrance. We observed
that the beam formed by the ConDuct electrode has a very low divergence (less
than 1°) and persists for long distances in vacuum. Intrigued by these
properties, we incorporated this electrode into a novel atmosphere-to-vacuum ion
transmission interface, and devised a technique for evaluating its performance
relative to the commercial reference interfaces that contain heated metal
capillaries. We determined that our new interface transmits at least 400 times
more ions than the commercial Thermo LCQ DECA XP atmosphere-to-vacuum interface
and 2 to 3 times more than the commercial interface in the Thermo Velos Orbitrap
and the Q Exactive mass spectrometers. We conclude that it might be possible to
optimize the properties of the transmitted ions further by manufacturing ConDuct
inlet electrodes from metal rather than conductive plastic and by determining the
optimum angle of channel divergence and channel length.
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