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10.1371/journal.pone.0121527 http://scihub22266oqcxt.onion/10.1371/journal.pone.0121527 C4361055!4361055!25775126     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2015 ; 10 (3): ä Nephropedia Template TP {{tp|p=25775126|t=2015. Viral Infection of Human Lung Macrophages Increases PDL1 Expression via IFN? |pdf=|usr=}}{{25775126}} gab.com Text Viral Infection of Human Lung Macrophages Increases PDL1 Expression via IFN JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25775126 #FOAvip Lung macrophages are an important defence against respiratory viral infection and recent work has demonstrated that influenza-induced macrophage PDL1 expression in the murine lung leads to rapid modulation of CD8+ T cell responses via the PD1 receptor. This PD1/PDL1 pathway may downregulate acute inflammatory responses to prevent tissue damage. The aim of this study was to investigate the mechanisms of PDL1 regulation by human macrophages in response to viral infection. Ex-vivo viral infection models using influenza and RSV were established in human lung explants, isolated lung macrophages and monocyte-derived macrophages (MDM) and analysed by flow cytometry and RT-PCR. Incubation of lung explants, lung macrophages and MDM with X31 resulted in mean cellular infection rates of 18%, 18% and 29% respectively. Viral infection significantly increased cell surface expression of PDL1 on explant macrophages, lung macrophages and MDM but not explant epithelial cells. Infected MDM induced IFN? release from autologous CD8+ T cells, an effect enhanced by PDL1 blockade. We observed increases in PDL1 mRNA and IFN? mRNA and protein release by MDM in response to influenza infection. Knockdown of IFN? by siRNA, resulted in a 37.5% reduction in IFN? gene expression in response to infection, and a significant decrease in PDL1 mRNA. Furthermore, when MDM were incubated with IFN?, this cytokine caused increased expression of PDL1 mRNA. These data indicate that human macrophage PDL1 expression modulates CD8+ cell IFN? release in response to virus and that this expression is regulated by autologous IFN? production. Twit Text FOAVip Viral Infection of Human Lung Macrophages Increases PDL1 Expression via IFN ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25775126 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25775126 #FOAvip English Wikipedia <ref>{{Cite pmid|25775126}}</ref> Viral Infection of Human Lung Macrophages Increases PDL1 Expression via IFN? #MMPMID25775126 Staples KJ; Nicholas B; McKendry RT; Spalluto CM; Wallington JC; Bragg CW; Robinson EC; Martin K; Djukanovi? R; Wilkinson TMA PLoS One 2015[]; 10 (3): ä PMID25775126show ga Lung macrophages are an important defence against respiratory viral infection and recent work has demonstrated that influenza-induced macrophage PDL1 expression in the murine lung leads to rapid modulation of CD8+ T cell responses via the PD1 receptor. This PD1/PDL1 pathway may downregulate acute inflammatory responses to prevent tissue damage. The aim of this study was to investigate the mechanisms of PDL1 regulation by human macrophages in response to viral infection. Ex-vivo viral infection models using influenza and RSV were established in human lung explants, isolated lung macrophages and monocyte-derived macrophages (MDM) and analysed by flow cytometry and RT-PCR. Incubation of lung explants, lung macrophages and MDM with X31 resulted in mean cellular infection rates of 18%, 18% and 29% respectively. Viral infection significantly increased cell surface expression of PDL1 on explant macrophages, lung macrophages and MDM but not explant epithelial cells. Infected MDM induced IFN? release from autologous CD8+ T cells, an effect enhanced by PDL1 blockade. We observed increases in PDL1 mRNA and IFN? mRNA and protein release by MDM in response to influenza infection. Knockdown of IFN? by siRNA, resulted in a 37.5% reduction in IFN? gene expression in response to infection, and a significant decrease in PDL1 mRNA. Furthermore, when MDM were incubated with IFN?, this cytokine caused increased expression of PDL1 mRNA. These data indicate that human macrophage PDL1 expression modulates CD8+ cell IFN? release in response to virus and that this expression is regulated by autologous IFN? production. äViral Infection of Human Lung Macrophages Increases PDL1 Expression vi(epmc).pdf DeepDyve Pubget Overpricing