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10.1038/nature14237 http://scihub22266oqcxt.onion/10.1038/nature14237 C4359072!4359072!25707795     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2015 ; 519 (7542): 193-8 Nephropedia Template TP {{tp|p=25707795|t=2015. Integrase-mediated spacer acquisition during CRISPR?Cas adaptive immunity |pdf=|usr=}}{{25707795}} gab.com Text Integrase-mediated spacer acquisition during CRISPR Cas adaptive immunity JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=25707795 #FOAvip Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1?Cas2 complex mediates spacer acquisition in vivo, but the molecular mechanism of this process is unknown. Here we show that the purified Cas1?Cas2 complex integrates oligonucleotide DNA substrates into acceptor DNA to yield products similar to those generated by retroviral integrases and transposases. Cas1 is the catalytic subunit, whereas Cas2 substantially increases integration activity. Protospacer DNA with free 3'-OH ends and supercoiled target DNA are required, and integration occurs preferentially at the ends of CRISPR repeats and at sequences adjacent to cruciform structures abutting A-T rich regions, similar to the CRISPR leader sequence. Our results demonstrate the Cas1?Cas2 complex to be the minimal machinery that catalyzes spacer DNA acquisition and explain the significance of CRISPR repeats in providing sequence and structural specificity for Cas1?Cas2-mediated adaptive immunity. Twit Text FOAVip Integrase-mediated spacer acquisition during CRISPR Cas adaptive immunity ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=25707795 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25707795 #FOAvip English Wikipedia <ref>{{Cite pmid|25707795}}</ref> Integrase-mediated spacer acquisition during CRISPR?Cas adaptive immunity #MMPMID25707795 Nuņez JK; Lee AS; Engelman A; Doudna JA Nature 2015[Mar]; 519 (7542): 193-8 PMID25707795show ga Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1?Cas2 complex mediates spacer acquisition in vivo, but the molecular mechanism of this process is unknown. Here we show that the purified Cas1?Cas2 complex integrates oligonucleotide DNA substrates into acceptor DNA to yield products similar to those generated by retroviral integrases and transposases. Cas1 is the catalytic subunit, whereas Cas2 substantially increases integration activity. Protospacer DNA with free 3'-OH ends and supercoiled target DNA are required, and integration occurs preferentially at the ends of CRISPR repeats and at sequences adjacent to cruciform structures abutting A-T rich regions, similar to the CRISPR leader sequence. Our results demonstrate the Cas1?Cas2 complex to be the minimal machinery that catalyzes spacer DNA acquisition and explain the significance of CRISPR repeats in providing sequence and structural specificity for Cas1?Cas2-mediated adaptive immunity. äIntegrase-mediated spacer acquisition during CRISPR?Cas adaptive immun(epmc).pdf DeepDyve Pubget Overpricing