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10.1128/AEM.04023-14 http://scihub22266oqcxt.onion/10.1128/AEM.04023-14 C4357945!4357945!25636838     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25636838&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Environ+Microbiol 2015 ; 81 (7): 2506-14 Nephropedia Template TP {{tp|p=25636838|t=2015. Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System |pdf=|usr=}}{{25636838}} gab.com Text Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System JO: Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25636838 #FOAvip An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%. Twit Text FOAVip Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System ????Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25636838 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25636838 #FOAvip English Wikipedia <ref>{{Cite pmid|25636838}}</ref> Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System #MMPMID25636838 Jiang Y; Chen B; Duan C; Sun B; Yang J; Yang S Appl Environ Microbiol 2015[Apr]; 81 (7): 2506-14 PMID25636838show ga An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%. äMultigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 S(epmc).pdf DeepDyve Pubget Overpricing