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10.1158/2326-6066.CIR-14-0001 http://scihub22266oqcxt.onion/10.1158/2326-6066.CIR-14-0001 C4351150!4351150!25576336     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cancer+Immunol+Res 2015 ; 3 (3): 228-35 Nephropedia Template TP {{tp|p=25576336|t=2015. Peptide/MHC tetramer-based sorting of CD8+ T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire |pdf=|usr=}}{{25576336}} gab.com Text Peptide/MHC tetramer-based sorting of CD8+ T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire JO: Cancer Immunol Res http://www.kidney.de/pget.php?&tw=1&pmid=25576336 #FOAvip Testing of T cell-based cancer therapeutics often involves measuring cancer antigen-specific T-cell populations with the assumption that they arise from in vivo clonal expansion. This analysis, using peptide/MHC tetramers, is often ambiguous.From a leukemia cell line, we identified a CDK4-derived peptide epitope, UNC-CDK4-1 (ALTPVVVTL) that bound HLA-A*02:01 with high affinity and could induce CD8+ T-cell responses in vitro. We identified UNC-CDK4-1/HLA-A*02:01 tetramer+ populations in 3 of 6 patients with acute myeloid leukemia who had undergone allogeneic stem cell transplant (SCT).Using tetramer-based, single-cell sorting and T-cell receptor ? (TCR?) sequencing we identified recurrent UNC-CDK4-1 tetramer-associated TCR? clonotypes in a patient with a UNC-CDK4-1 tetramer+ population, suggesting in vivo T-cell expansion to UNC-CDK4-1. In parallel, we measured the patient's TCR? repertoire and found it to be highly restricted / oligoclonal. The UNC-CDK4-1 tetramer-associated TCR? clonotypes represented >17% of the entire TCR? repertoire ? far in excess of the UNC-CDK4-1 tetramer+ frequency, indicating that the recurrent TCR? clonotypes identified from UNC-CDK-4-1 tetramer+ cells were likely a consequence of the extremely constrained T-cell repertoire in the patient and not in vivo UNC-CDK4-1-driven clonal T-cell expansion.Mapping recurrent TCR? clonotype sequences onto TCR? repertoires can help confirm or refute antigen-specific T-cell expansion in vivo. Twit Text FOAVip Peptide/MHC tetramer-based sorting of CD8+ T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire ????Cancer Immunol Res http://www.kidney.de/pget.php?&tw=1&pmid=25576336 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25576336 #FOAvip English Wikipedia <ref>{{Cite pmid|25576336}}</ref> Peptide/MHC tetramer-based sorting of CD8+ T cells to a leukemia antigen yields clonotypes drawn nonspecifically from an underlying restricted repertoire #MMPMID25576336 Hunsucker SA; McGary CS; Vincent BG; Enyenihi AA; Waugh JP; McKinnon KP; Bixby LM; Ropp PA; Coghill JM; Wood WA; Gabriel DA; Sarantopoulos S; Shea TC; Serody JS; Alatrash G; Rodriguez-Cruz T; Lizée G; Buntzman AS; Frelinger JA; Glish GL; Armistead PM Cancer Immunol Res 2015[Mar]; 3 (3): 228-35 PMID25576336show ga Testing of T cell-based cancer therapeutics often involves measuring cancer antigen-specific T-cell populations with the assumption that they arise from in vivo clonal expansion. This analysis, using peptide/MHC tetramers, is often ambiguous.From a leukemia cell line, we identified a CDK4-derived peptide epitope, UNC-CDK4-1 (ALTPVVVTL) that bound HLA-A*02:01 with high affinity and could induce CD8+ T-cell responses in vitro. We identified UNC-CDK4-1/HLA-A*02:01 tetramer+ populations in 3 of 6 patients with acute myeloid leukemia who had undergone allogeneic stem cell transplant (SCT).Using tetramer-based, single-cell sorting and T-cell receptor ? (TCR?) sequencing we identified recurrent UNC-CDK4-1 tetramer-associated TCR? clonotypes in a patient with a UNC-CDK4-1 tetramer+ population, suggesting in vivo T-cell expansion to UNC-CDK4-1. In parallel, we measured the patient's TCR? repertoire and found it to be highly restricted / oligoclonal. The UNC-CDK4-1 tetramer-associated TCR? clonotypes represented >17% of the entire TCR? repertoire ? far in excess of the UNC-CDK4-1 tetramer+ frequency, indicating that the recurrent TCR? clonotypes identified from UNC-CDK-4-1 tetramer+ cells were likely a consequence of the extremely constrained T-cell repertoire in the patient and not in vivo UNC-CDK4-1-driven clonal T-cell expansion.Mapping recurrent TCR? clonotype sequences onto TCR? repertoires can help confirm or refute antigen-specific T-cell expansion in vivo. äPeptide/MHC tetramer-based sorting of CD8+ T cells to a leukemia antig(epmc).pdf DeepDyve Pubget Overpricing