Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1074/mcp.M114.044016 http://scihub22266oqcxt.onion/10.1074/mcp.M114.044016 C4349989!4349989!25595278     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Proteomics 2015 ; 14 (3): 707-23 Nephropedia Template TP {{tp|p=25595278|t=2015. Monitoring Interactions and Dynamics of Endogenous Beta-catenin With Intracellular Nanobodies in Living Cells* |pdf=|usr=}}{{25595278}} gab.com Text Monitoring Interactions and Dynamics of Endogenous Beta-catenin With Intracellular Nanobodies in Living Cells* JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25595278 #FOAvip ?-catenin is the key component of the canonical Wnt pathway and plays a crucial role in a multitude of developmental and homeostatic processes. The different tasks of ?-catenin are orchestrated by its subcellular localization and participation in multiprotein complexes. To gain a better understanding of ?-catenin's role in living cells we have generated a new set of single domain antibodies, referred to as nanobodies, derived from heavy chain antibodies of camelids. We selected nanobodies recognizing the N-terminal, core or C-terminal domain of ?-catenin and applied these new high-affinity binders as capture molecules in sandwich immunoassays and co-immunoprecipitations of endogenous ?-catenin complexes. In addition, we engineered intracellularly functional anti-?-catenin chromobodies by combining the binding moieties of the nanobodies with fluorescent proteins. For the first time, we were able to visualize the subcellular localization and nuclear translocation of endogenous ?-catenin in living cells using these chromobodies. Moreover, the chromobody signal allowed us to trace the accumulation of diffusible, hypo-phosphorylated ?-catenin in response to compound treatment in real time using High Content Imaging. The anti-?-catenin nanobodies and chromobodies characterized in this study are versatile tools that enable a novel and unique approach to monitor the dynamics of subcellular ?-catenin in biochemical and cell biological assays. Twit Text FOAVip Monitoring Interactions and Dynamics of Endogenous Beta-catenin With Intracellular Nanobodies in Living Cells* ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25595278 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25595278 #FOAvip English Wikipedia <ref>{{Cite pmid|25595278}}</ref> Monitoring Interactions and Dynamics of Endogenous Beta-catenin With Intracellular Nanobodies in Living Cells* #MMPMID25595278 Traenkle B; Emele F; Anton R; Poetz O; Haeussler RS; Maier J; Kaiser PD; Scholz AM; Nueske S; Buchfellner A; Romer T; Rothbauer U Mol Cell Proteomics 2015[Mar]; 14 (3): 707-23 PMID25595278show ga ?-catenin is the key component of the canonical Wnt pathway and plays a crucial role in a multitude of developmental and homeostatic processes. The different tasks of ?-catenin are orchestrated by its subcellular localization and participation in multiprotein complexes. To gain a better understanding of ?-catenin's role in living cells we have generated a new set of single domain antibodies, referred to as nanobodies, derived from heavy chain antibodies of camelids. We selected nanobodies recognizing the N-terminal, core or C-terminal domain of ?-catenin and applied these new high-affinity binders as capture molecules in sandwich immunoassays and co-immunoprecipitations of endogenous ?-catenin complexes. In addition, we engineered intracellularly functional anti-?-catenin chromobodies by combining the binding moieties of the nanobodies with fluorescent proteins. For the first time, we were able to visualize the subcellular localization and nuclear translocation of endogenous ?-catenin in living cells using these chromobodies. Moreover, the chromobody signal allowed us to trace the accumulation of diffusible, hypo-phosphorylated ?-catenin in response to compound treatment in real time using High Content Imaging. The anti-?-catenin nanobodies and chromobodies characterized in this study are versatile tools that enable a novel and unique approach to monitor the dynamics of subcellular ?-catenin in biochemical and cell biological assays. äMonitoring Interactions and Dynamics of Endogenous Beta-catenin With I(epmc).pdf DeepDyve Pubget Overpricing