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10.1038/cr.2015.17 http://scihub22266oqcxt.onion/10.1038/cr.2015.17 C4349253!4349253!25656846     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Res 2015 ; 25 (3): 370-85 Nephropedia Template TP {{tp|p=25656846|t=2015. EWI-2 negatively regulates TGF-? signaling leading to altered melanoma growth and metastasis |pdf=|usr=}}{{25656846}} gab.com Text EWI-2 negatively regulates TGF- signaling leading to altered melanoma growth and metastasis JO: Cell Res http://www.kidney.de/pget.php?&tw=1&pmid=25656846 #FOAvip In normal melanocytes, TGF-? signaling has a cytostatic effect. However, in primary melanoma cells, TGF-?-induced cytostasis is diminished, thus allowing melanoma growth. Later, a second phase of TGF-? signaling supports melanoma EMT-like changes, invasion and metastasis. In parallel with these ?present-absent-present? TGF-? signaling phases, cell surface protein EWI motif-containing protein 2 (EWI-2 or IgSF8) is ?absent-present-absent? in melanocytes, primary melanoma, and metastatic melanoma, respectively, suggesting that EWI-2 may serve as a negative regulator of TGF-? signaling. Using melanoma cell lines and melanoma short-term cultures, we performed RNAi and overexpression experiments and found that EWI-2 negatively regulates TGF-? signaling and its downstream events including cytostasis (in vitro and in vivo), EMT-like changes, cell migration, CD271-dependent invasion, and lung metastasis (in vivo). When EWI-2 is present, it associates with cell surface tetraspanin proteins CD9 and CD81 ? molecules not previously linked to TGF-? signaling. Indeed, when associated with EWI-2, CD9 and CD81 are sequestered and have no impact on T?R2-T?R1 association or TGF-? signaling. However, when EWI-2 is knocked down, CD9 and CD81 become available to provide critical support for T?R2-T?R1 association, thus markedly elevating TGF-? signaling. Consequently, all of those TGF-?-dependent functions specifically arising due to EWI-2 depletion are reversed by blocking or depleting cell surface tetraspanin proteins CD9 or CD81. These results provide new insights into regulation of TGF-? signaling in melanoma, uncover new roles for tetraspanins CD9 and CD81, and strongly suggest that EWI-2 could serve as a favorable prognosis indicator for melanoma patients. Twit Text FOAVip EWI-2 negatively regulates TGF- signaling leading to altered melanoma growth and metastasis ????Cell Res http://www.kidney.de/pget.php?&tw=1&pmid=25656846 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25656846 #FOAvip English Wikipedia <ref>{{Cite pmid|25656846}}</ref> EWI-2 negatively regulates TGF-? signaling leading to altered melanoma growth and metastasis #MMPMID25656846 Wang HX; Sharma C; Knoblich K; Granter SR; Hemler ME Cell Res 2015[Mar]; 25 (3): 370-85 PMID25656846show ga In normal melanocytes, TGF-? signaling has a cytostatic effect. However, in primary melanoma cells, TGF-?-induced cytostasis is diminished, thus allowing melanoma growth. Later, a second phase of TGF-? signaling supports melanoma EMT-like changes, invasion and metastasis. In parallel with these ?present-absent-present? TGF-? signaling phases, cell surface protein EWI motif-containing protein 2 (EWI-2 or IgSF8) is ?absent-present-absent? in melanocytes, primary melanoma, and metastatic melanoma, respectively, suggesting that EWI-2 may serve as a negative regulator of TGF-? signaling. Using melanoma cell lines and melanoma short-term cultures, we performed RNAi and overexpression experiments and found that EWI-2 negatively regulates TGF-? signaling and its downstream events including cytostasis (in vitro and in vivo), EMT-like changes, cell migration, CD271-dependent invasion, and lung metastasis (in vivo). When EWI-2 is present, it associates with cell surface tetraspanin proteins CD9 and CD81 ? molecules not previously linked to TGF-? signaling. Indeed, when associated with EWI-2, CD9 and CD81 are sequestered and have no impact on T?R2-T?R1 association or TGF-? signaling. However, when EWI-2 is knocked down, CD9 and CD81 become available to provide critical support for T?R2-T?R1 association, thus markedly elevating TGF-? signaling. Consequently, all of those TGF-?-dependent functions specifically arising due to EWI-2 depletion are reversed by blocking or depleting cell surface tetraspanin proteins CD9 or CD81. These results provide new insights into regulation of TGF-? signaling in melanoma, uncover new roles for tetraspanins CD9 and CD81, and strongly suggest that EWI-2 could serve as a favorable prognosis indicator for melanoma patients. äEWI-2 negatively regulates TGF-? signaling leading to altered melanoma(epmc).pdf DeepDyve Pubget Overpricing