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10.1378/chest.14-0380 http://scihub22266oqcxt.onion/10.1378/chest.14-0380 C4347535!4347535!25411763     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chest 2015 ; 147 (3): 771-7 Nephropedia Template TP {{tp|p=25411763|t=2015. Antibody ?PEP13h Reacts With Lymphangioleiomyomatosis Cells in Lung Nodules |pdf=|usr=}}{{25411763}} gab.com Text Antibody PEP13h Reacts With Lymphangioleiomyomatosis Cells in Lung Nodules JO: Chest http://www.kidney.de/pget.php?&tw=1&pmid=25411763 #FOAvip BACKGROUND:: Lymphangioleiomyomatosis (LAM) is characterized by the proliferation in the lung, axial lymphatics (eg, lymphangioleiomyomas), and kidney (eg, angiomyolipomas) of abnormal smooth muscle-like LAM cells, which express melanoma antigens such as Pmel17/gp100 and have dysfunctional tumor suppressor tuberous sclerosis complex (TSC) genes TSC2 or TSC1. Histopathologic diagnosis of LAM in lung specimens is based on identification of the Pmel17 protein with the monoclonal antibody HMB-45. METHODS:: We compared the sensitivity of HMB-45 to that of antipeptide antibody ?PEP13h, which reacts with a C-terminal peptide of Pmel17. LAM lung nodules were laser-capture microdissected to identify proteins by Western blotting. RESULTS:: HMB-45 recognized approximately 25% of LAM cells within the LAM lung nodules, whereas ?PEP13h identified > 82% of LAM cells within these structures in approximately 90% of patients. Whereas HMB-45 reacted with epithelioid but not with spindle-shaped LAM cells, ?PEP13h identified both spindle-shaped and epithelioid LAM cells, providing greater sensitivity for detection of all types of LAM cells. HMB-45 recognized Pmel17 in premelanosomal organelles; ?PEP13h recognized proteins in the cytoplasm as well as in premelanosomal organelles. Both antibodies recognized a Pmel17 variant of approximately 50 kDa. CONCLUSIONS:: Based on its sensitivity and specificity, ?PEP13h may be useful in the diagnosis of LAM and more sensitive than HMB-45. Twit Text FOAVip Antibody PEP13h Reacts With Lymphangioleiomyomatosis Cells in Lung Nodules ????Chest http://www.kidney.de/pget.php?&tw=1&pmid=25411763 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25411763 #FOAvip English Wikipedia <ref>{{Cite pmid|25411763}}</ref> Antibody ?PEP13h Reacts With Lymphangioleiomyomatosis Cells in Lung Nodules #MMPMID25411763 Valencia JC; Steagall WK; Zhang Y; Fetsch P; Abati A; Tsukada K; Billings E; Hearing VJ; Yu ZX; Pacheco-Rodriguez G; Moss J Chest 2015[Mar]; 147 (3): 771-7 PMID25411763show ga BACKGROUND:: Lymphangioleiomyomatosis (LAM) is characterized by the proliferation in the lung, axial lymphatics (eg, lymphangioleiomyomas), and kidney (eg, angiomyolipomas) of abnormal smooth muscle-like LAM cells, which express melanoma antigens such as Pmel17/gp100 and have dysfunctional tumor suppressor tuberous sclerosis complex (TSC) genes TSC2 or TSC1. Histopathologic diagnosis of LAM in lung specimens is based on identification of the Pmel17 protein with the monoclonal antibody HMB-45. METHODS:: We compared the sensitivity of HMB-45 to that of antipeptide antibody ?PEP13h, which reacts with a C-terminal peptide of Pmel17. LAM lung nodules were laser-capture microdissected to identify proteins by Western blotting. RESULTS:: HMB-45 recognized approximately 25% of LAM cells within the LAM lung nodules, whereas ?PEP13h identified > 82% of LAM cells within these structures in approximately 90% of patients. Whereas HMB-45 reacted with epithelioid but not with spindle-shaped LAM cells, ?PEP13h identified both spindle-shaped and epithelioid LAM cells, providing greater sensitivity for detection of all types of LAM cells. HMB-45 recognized Pmel17 in premelanosomal organelles; ?PEP13h recognized proteins in the cytoplasm as well as in premelanosomal organelles. Both antibodies recognized a Pmel17 variant of approximately 50 kDa. CONCLUSIONS:: Based on its sensitivity and specificity, ?PEP13h may be useful in the diagnosis of LAM and more sensitive than HMB-45. äAntibody ?PEP13h Reacts With Lymphangioleiomyomatosis Cells in Lung No(epmc).pdf DeepDyve Pubget Overpricing