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2015 ; 14
(3
): 228-40
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Role of Tec1 in the development, architecture, and integrity of sexual biofilms
of Candida albicans
#MMPMID25556183
Daniels KJ
; Srikantha T
; Pujol C
; Park YN
; Soll DR
Eukaryot Cell
2015[Mar]; 14
(3
): 228-40
PMID25556183
show ga
MTL-homozygous ( A: / A: or ?/?) white cells form a complex sexual biofilm that
exhibits the same architecture as that of MTL-heterozygous ( A: /?) pathogenic
biofilms. However, the former is regulated by the mitogen-activated protein (MAP)
kinase pathway, while the latter is regulated by the Ras1/cyclic AMP (cAMP)
pathway. We previously demonstrated that in the formation of an MTL-homozygous,
mature (48 h) sexual biofilm in RPMI 1640 medium, the MAP kinase pathway targets
Tec1 rather than Cph1, the latter of which is the target of the same pathway, but
for the opaque cell mating response. Here we continued our analysis of the role
of Tec1 by comparing the effects of deleting TEC1 on initial adhesion to silicone
elastomer, high-resolution confocal microscopy assessments of the stages and
cellular phenotypes during the 48 h of biofilm development, human white cell
penetration, and biofilm fragility. We show that although Tec1 plays only a minor
role in initial adhesion to the silicone elastomer, it does play a major role in
the growth of the basal yeast cell polylayer, vertical extension of hyphae and
matrix deposition in the upper portion of the biofilm, final biofilm thickness,
penetrability of human white blood cells, and final biofilm integrity (i.e.,
resistance to fluid flow). These results provide a more detailed description of
normal biofilm development and architecture and confirm the central role played
by the transcription factor Tec1 in the biofilm model employed here.
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