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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Proc+Biol+Sci
2015 ; 282
(1803
): 20142773
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English Wikipedia
Who is the puppet master? Replication of a parasitic wasp-associated virus
correlates with host behaviour manipulation
#MMPMID25673681
Dheilly NM
; Maure F
; Ravallec M
; Galinier R
; Doyon J
; Duval D
; Leger L
; Volkoff AN
; Missé D
; Nidelet S
; Demolombe V
; Brodeur J
; Gourbal B
; Thomas F
; Mitta G
Proc Biol Sci
2015[Mar]; 282
(1803
): 20142773
PMID25673681
show ga
Many parasites modify their host behaviour to improve their own transmission and
survival, but the proximate mechanisms remain poorly understood. An original
model consists of the parasitoid Dinocampus coccinellae and its coccinellid host,
Coleomegilla maculata; during the behaviour manipulation, the parasitoid is not
in contact with its host anymore. We report herein the discovery and
characterization of a new RNA virus of the parasitoid (D. coccinellae paralysis
virus, DcPV). Using a combination of RT-qPCR and transmission electron
microscopy, we demonstrate that DcPV is stored in the oviduct of parasitoid
females, replicates in parasitoid larvae and is transmitted to the host during
larval development. Next, DcPV replication in the host's nervous tissue induces a
severe neuropathy and antiviral immune response that correlate with the paralytic
symptoms characterizing the behaviour manipulation. Remarkably, virus clearance
correlates with recovery of normal coccinellid behaviour. These results provide
evidence that changes in ladybeetle behaviour most likely result from DcPV
replication in the cerebral ganglia rather than by manipulation by the
parasitoid. This offers stimulating prospects for research on parasitic
manipulation by suggesting for the first time that behaviour manipulation could
be symbiont-mediated.