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10.1128/AEM.02863-14 http://scihub22266oqcxt.onion/10.1128/AEM.02863-14 C4345377!4345377!25595761     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Environ+Microbiol 2015 ; 81 (6): 2149-55 Nephropedia Template TP {{tp|p=25595761|t=2015. Counting Viruses and Bacteria in Photosynthetic Microbial Mats |pdf=|usr=}}{{25595761}} gab.com Text Counting Viruses and Bacteria in Photosynthetic Microbial Mats JO: Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25595761 #FOAvip Viral abundances in benthic environments are the highest found in aquatic systems. Photosynthetic microbial mats represent benthic environments with high microbial activity and possibly high viral densities, yet viral abundances have not been examined in such systems. Existing extraction procedures typically used in benthic viral ecology were applied to the complex matrix of microbial mats but were found to inefficiently extract viruses. Here, we present a method for extraction and quantification of viruses from photosynthetic microbial mats using epifluorescence microscopy (EFM) and flow cytometry (FCM). A combination of EDTA addition, probe sonication, and enzyme treatment applied to a glutaraldehyde-fixed sample resulted in a substantially higher viral (5- to 33-fold) extraction efficiency and reduced background noise compared to previously published methods. Using this method, it was found that in general, intertidal photosynthetic microbial mats harbor very high viral abundances (2.8 × 1010 ± 0.3 × 1010 g?1) compared with benthic habitats (107 to 109 g?1). This procedure also showed 4.5- and 4-fold-increased efficacies of extraction of viruses and bacteria, respectively, from intertidal sediments, allowing a single method to be used for the microbial mat and underlying sediment. Twit Text FOAVip Counting Viruses and Bacteria in Photosynthetic Microbial Mats ????Appl Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25595761 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25595761 #FOAvip English Wikipedia <ref>{{Cite pmid|25595761}}</ref> Counting Viruses and Bacteria in Photosynthetic Microbial Mats #MMPMID25595761 Carreira C; Staal M; Middelboe M; Brussaard CPD Appl Environ Microbiol 2015[Mar]; 81 (6): 2149-55 PMID25595761show ga Viral abundances in benthic environments are the highest found in aquatic systems. Photosynthetic microbial mats represent benthic environments with high microbial activity and possibly high viral densities, yet viral abundances have not been examined in such systems. Existing extraction procedures typically used in benthic viral ecology were applied to the complex matrix of microbial mats but were found to inefficiently extract viruses. Here, we present a method for extraction and quantification of viruses from photosynthetic microbial mats using epifluorescence microscopy (EFM) and flow cytometry (FCM). A combination of EDTA addition, probe sonication, and enzyme treatment applied to a glutaraldehyde-fixed sample resulted in a substantially higher viral (5- to 33-fold) extraction efficiency and reduced background noise compared to previously published methods. Using this method, it was found that in general, intertidal photosynthetic microbial mats harbor very high viral abundances (2.8 × 1010 ± 0.3 × 1010 g?1) compared with benthic habitats (107 to 109 g?1). This procedure also showed 4.5- and 4-fold-increased efficacies of extraction of viruses and bacteria, respectively, from intertidal sediments, allowing a single method to be used for the microbial mat and underlying sediment. äCounting Viruses and Bacteria in Photosynthetic Microbial Mats (epmc).pdf DeepDyve Pubget Overpricing