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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Nat+Methods 2015 ; 12 (3): 215-8 Nephropedia Template TP
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Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy #MMPMID25581799
Paez Segala MG; Sun MG; Shtengel G; Viswanathan S; Baird MA; Macklin JJ; Patel R; Allen JR; Howe ES; Piszczek G; Hess HF; Davidson MW; Wang Y; Looger LL
Nat Methods 2015[Mar]; 12 (3): 215-8 PMID25581799show ga
Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluorescent protein that function normally in heavily fixed (0.5?1% OsO4), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.