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Chikungunya virus RNA and antibody testing at a National Reference Laboratory
since the emergence of Chikungunya virus in the Americas
#MMPMID25540275
Prince HE
; Seaton BL
; Matud JL
; Batterman HJ
Clin Vaccine Immunol
2015[Mar]; 22
(3
): 291-7
PMID25540275
show ga
Since first reported in the Americas in December 2013, chikungunya virus (CHIKV)
infections have been documented in travelers returning from the Caribbean, with
many cases identified by CHIKV antibody and/or RNA testing at our laboratory. We
used our large data set to characterize the relationship between antibody titers
and RNA detection and to estimate IgM persistence. CHIKV RNA was measured by
nucleic acid amplification and CHIKV IgG/IgM by indirect immunofluorescence. Of
the 1,306 samples submitted for RNA testing in January through September 2014,
393 (30%) were positive; for 166 RNA-positive samples, CHIKV antibody testing was
also ordered, and 84% were antibody negative. Of the 6,971 sera submitted for
antibody testing in January through September 2014, 1,811 (26%) were IgM
positive; 1,461 IgM positives (81%) were also IgG positive. The relationship
between the CHIKV antibody titers and RNA detection was evaluated using 376
IgM-positive samples (138 with RNA testing ordered and 238 deidentified and
tested for RNA). RNA detection showed no significant association with the IgM
titer but was inversely related to the IgG titer; 63% of the IgG negative sera
were RNA positive, compared to 36% of sera with low IgG titers (1:10 to 1:80) and
16% with IgG titers of ?1:160. Using second-sample results from 62
seroconverters, we estimated that CHIKV IgM persists for 110 days (95% confidence
interval, 78 to 150 days) after the initial antibody-negative sample. These
findings indicate that (i) RNA detection is more sensitive than antibody
detection early in CHIKV infection, (ii) in the absence of RNA results, the IgG
titer of the IgM-positive samples may be a useful surrogate for viremia, and
(iii) CHIKV IgM persists for approximately 4 months after symptom onset.
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