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2015 ; 21
(3
): 252-61
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Suppression of TRPM7 inhibits proliferation, migration, and invasion of malignant
human glioma cells
#MMPMID25438992
Leng TD
; Li MH
; Shen JF
; Liu ML
; Li XB
; Sun HW
; Branigan D
; Zeng Z
; Si HF
; Li J
; Chen J
; Xiong ZG
CNS Neurosci Ther
2015[Mar]; 21
(3
): 252-61
PMID25438992
show ga
BACKGROUND: Glioblastoma multiforme (GBM) is the most common and aggressive
primary brain tumor with a dismal prognosis. Despite intensive study on tumor
biology, the underlying mechanisms of the unlimited proliferation and progressive
local invasion are still poorly understood, and no effective treatment has been
developed for GBM patients. AIMS: We determine the role of TRPM7 channels in the
growth, migration, and infiltration of malignant glioma cells. METHODS: Using a
combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated
the expression of functional TRPM7 channels of A172 cells, a human glioma cell
line, as well as in human glioma tissues. Furthermore, we evaluated the role of
TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell
migration and invasion assays. RESULTS: We showed the expression of functional
TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7
expression with TRPM7-siRNA dramatically reduced the proliferation, migration,
and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with
2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA.
CONCLUSION: We demonstrate that human glioma cells express functional TRPM7
channel and that activation of this channel plays an important role in the
proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel
may represent a novel and promising target for therapeutic intervention of
malignant glioma.
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