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10.1261/rna.049098.114 http://scihub22266oqcxt.onion/10.1261/rna.049098.114 C4338334!4338334!25605965     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25605965&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA 2015 ; 21 (3): 375-84 Nephropedia Template TP {{tp|p=25605965|t=2015. A deeply conserved, noncanonical miRNA hosted by ribosomal DNA |pdf=|usr=}}{{25605965}} gab.com Text A deeply conserved, noncanonical miRNA hosted by ribosomal DNA JO: RNA http://www.kidney.de/pget.php?&tw=1&pmid=25605965 #FOAvip Advances in small RNA sequencing technologies and comparative genomics have fueled comprehensive microRNA (miRNA) gene annotations in humans and model organisms. Although new miRNAs continue to be discovered in recent years, these have universally been lowly expressed, recently evolved, and of debatable endogenous activity, leading to the general assumption that virtually all biologically important miRNAs have been identified. Here, we analyzed small RNAs that emanate from the highly repetitive rDNA arrays of Drosophila. In addition to endo-siRNAs derived from sense and antisense strands of the pre-rRNA sequence, we unexpectedly identified a novel, deeply conserved, noncanonical miRNA. Although this miRNA is widely expressed, this miRNA was not identified by previous studies due to bioinformatics filters removing such repetitive sequences. Deep-sequencing data provide clear evidence for specific processing with precisely defined 5? and 3? ends. Furthermore, we demonstrate that the mature miRNA species is incorporated in the effector complexes and has detectable trans regulatory activity. Processing of this miRNA requires Dicer-1, whereas the Drosha?Pasha complex is dispensable. The miRNA hairpin sequence is located in the internal transcribed spacer 1 region of rDNA and is highly conserved among Dipteran species that were separated from their common ancestor ?100 million years ago. Our results suggest that biologically active miRNA genes may remain unidentified even in well-studied organisms. Twit Text FOAVip A deeply conserved, noncanonical miRNA hosted by ribosomal DNA ????RNA http://www.kidney.de/pget.php?&tw=1&pmid=25605965 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25605965 #FOAvip English Wikipedia <ref>{{Cite pmid|25605965}}</ref> A deeply conserved, noncanonical miRNA hosted by ribosomal DNA #MMPMID25605965 Chak LL; Mohammed J; Lai EC; Tucker-Kellogg G; Okamura K RNA 2015[Mar]; 21 (3): 375-84 PMID25605965show ga Advances in small RNA sequencing technologies and comparative genomics have fueled comprehensive microRNA (miRNA) gene annotations in humans and model organisms. Although new miRNAs continue to be discovered in recent years, these have universally been lowly expressed, recently evolved, and of debatable endogenous activity, leading to the general assumption that virtually all biologically important miRNAs have been identified. Here, we analyzed small RNAs that emanate from the highly repetitive rDNA arrays of Drosophila. In addition to endo-siRNAs derived from sense and antisense strands of the pre-rRNA sequence, we unexpectedly identified a novel, deeply conserved, noncanonical miRNA. Although this miRNA is widely expressed, this miRNA was not identified by previous studies due to bioinformatics filters removing such repetitive sequences. Deep-sequencing data provide clear evidence for specific processing with precisely defined 5? and 3? ends. Furthermore, we demonstrate that the mature miRNA species is incorporated in the effector complexes and has detectable trans regulatory activity. Processing of this miRNA requires Dicer-1, whereas the Drosha?Pasha complex is dispensable. The miRNA hairpin sequence is located in the internal transcribed spacer 1 region of rDNA and is highly conserved among Dipteran species that were separated from their common ancestor ?100 million years ago. Our results suggest that biologically active miRNA genes may remain unidentified even in well-studied organisms. äA deeply conserved, noncanonical miRNA hosted by ribosomal DNA (epmc).pdf DeepDyve Pubget Overpricing