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10.1021/ja500327g http://scihub22266oqcxt.onion/10.1021/ja500327g C4333597!4333597!24802207     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Chem+Soc 2014 ; 136 (19): 7152-8 Nephropedia Template TP {{tp|p=24802207|t=2014. Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals |pdf=|usr=}}{{24802207}} gab.com Text Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals JO: J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=24802207 #FOAvip In cell and molecular biology, double-stranded circular DNA constructs, known as plasmids, are extensively used to express a gene of interest. These gene expression systems rely on a specific promoter region to drive the transcription of genes either constitutively (i.e., in a continually ?ON? state) or conditionally (i.e., in response to a specific transcription initiator). However, controlling plasmid-based expression with high spatial and temporal resolution in cellular environments and in multicellular organisms remains challenging. To overcome this limitation, we have site-specifically installed nucleobase-caging groups within a plasmid promoter region to enable optochemical control of transcription and, thus, gene expression, via photolysis of the caging groups. Through the light-responsive modification of plasmid-based gene expression systems, we have demonstrated optochemical activation of an exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light-induced overexpression of an endogenous signaling protein. Twit Text FOAVip Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals ????J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=24802207 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24802207 #FOAvip English Wikipedia <ref>{{Cite pmid|24802207}}</ref> Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals #MMPMID24802207 Hemphill J; Govan J; Uprety R; Tsang M; Deiters A J Am Chem Soc 2014[May]; 136 (19): 7152-8 PMID24802207show ga In cell and molecular biology, double-stranded circular DNA constructs, known as plasmids, are extensively used to express a gene of interest. These gene expression systems rely on a specific promoter region to drive the transcription of genes either constitutively (i.e., in a continually ?ON? state) or conditionally (i.e., in response to a specific transcription initiator). However, controlling plasmid-based expression with high spatial and temporal resolution in cellular environments and in multicellular organisms remains challenging. To overcome this limitation, we have site-specifically installed nucleobase-caging groups within a plasmid promoter region to enable optochemical control of transcription and, thus, gene expression, via photolysis of the caging groups. Through the light-responsive modification of plasmid-based gene expression systems, we have demonstrated optochemical activation of an exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light-induced overexpression of an endogenous signaling protein. äSite-Specific Promoter Caging Enables Optochemical Gene Activation in (epmc).pdf DeepDyve Pubget Overpricing