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10.1128/IAI.02759-14

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suck abstract from ncbi


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pmid25534938
      Infect+Immun 2015 ; 83 (3 ): 907-22
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  • Antisecretory factor peptide AF-16 inhibits the secreted autotransporter toxin-stimulated transcellular and paracellular passages of fluid in cultured human enterocyte-like cells #MMPMID25534938
  • Nicolas V ; Liévin-Le Moal V
  • Infect Immun 2015[Mar]; 83 (3 ): 907-22 PMID25534938 show ga
  • Both the endogenous antisecretory factor (AF) protein and peptide AF-16, which has a sequence that matches that of the active N-terminal region of AF, inhibit the increase in the epithelial transport of fluid and electrolytes induced by bacterial toxins in animal and ex vivo models. We conducted a study to investigate the inhibitory effect of peptide AF-16 against the increase of transcellular passage and paracellular permeability promoted by the secreted autotransporter toxin (Sat) in a cultured cellular model of the human intestinal epithelial barrier. Peptide AF-16 produced a concentration-dependent inhibition of the Sat-induced increase in the formation of fluid domes, in the mucosal-to-serosal passage of D-[1-(14)C]mannitol, and in the rearrangements in the distribution and protein expression of the tight junction (TJ)-associated proteins ZO-1 and occludin in cultured human enterocyte-like Caco-2/TC7 cell monolayers. In addition, we show that peptide AF-16 also inhibits the cholera toxin-induced increase of transcellular passage and the Clostridium difficile toxin-induced effects on paracellular permeability and TJ protein organization in Caco-2/TC7 cell monolayers. Treatment of cell monolayers by the lipid raft disorganizer methyl-?-cyclodextrin abolished the inhibitory activity of peptide AF-16 at the transcellular passage level and did not modify the effect of the peptide at the paracellular level.
  • |Amino Acid Sequence [MESH]
  • |Biological Transport/drug effects [MESH]
  • |Caco-2 Cells [MESH]
  • |Carbon Radioisotopes [MESH]
  • |Cholera Toxin/*antagonists & inhibitors/toxicity [MESH]
  • |Enterocytes/cytology/*drug effects/metabolism [MESH]
  • |Enterotoxins/*antagonists & inhibitors/toxicity [MESH]
  • |Humans [MESH]
  • |Kinetics [MESH]
  • |Mannitol/metabolism [MESH]
  • |Molecular Sequence Data [MESH]
  • |Neuropeptides/chemistry/*pharmacology [MESH]
  • |Peptides/chemistry/*pharmacology [MESH]
  • |Permeability/drug effects [MESH]
  • |Tight Junctions/drug effects/metabolism [MESH]


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