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10.1038/nchembio.1736 http://scihub22266oqcxt.onion/10.1038/nchembio.1736 C4333066!4333066!25643171     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2015 ; 11 (3): 207-13 Nephropedia Template TP {{tp|p=25643171|t=2015. Modular construction of mammalian gene circuits using TALE transcriptional repressors |pdf=|usr=}}{{25643171}} gab.com Text Modular construction of mammalian gene circuits using TALE transcriptional repressors JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=25643171 #FOAvip An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable parts. However, engineering of complex circuits in mammalian cells is currently limited by the availability of well-characterized and orthogonal transcriptional repressors. Here, we introduce a library of 26 reversible transcription activator-like effector repressors (TALERs) that bind newly designed hybrid promoters and exert transcriptional repression through steric hindrance of key transcriptional initiation elements. We demonstrate that using the input-output transfer curves of our TALERs enables accurate prediction of the behavior of modularly assembled TALER cascade and switch circuits. We also show that TALER switches employing feedback regulation exhibit improved accuracy for microRNA-based HeLa cancer cell classification versus HEK293 cells. Our TALER library is a valuable toolkit for modular engineering of synthetic circuits, enabling programmable manipulation of mammalian cells and helping elucidate design principles of coupled transcriptional and microRNA-mediated post-transcriptional regulation. Twit Text FOAVip Modular construction of mammalian gene circuits using TALE transcriptional repressors ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=25643171 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25643171 #FOAvip English Wikipedia <ref>{{Cite pmid|25643171}}</ref> Modular construction of mammalian gene circuits using TALE transcriptional repressors #MMPMID25643171 Li Y; Jiang Y; Chen H; Liao W; Li Z; Weiss R; Xie Z Nat Chem Biol 2015[Mar]; 11 (3): 207-13 PMID25643171show ga An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable parts. However, engineering of complex circuits in mammalian cells is currently limited by the availability of well-characterized and orthogonal transcriptional repressors. Here, we introduce a library of 26 reversible transcription activator-like effector repressors (TALERs) that bind newly designed hybrid promoters and exert transcriptional repression through steric hindrance of key transcriptional initiation elements. We demonstrate that using the input-output transfer curves of our TALERs enables accurate prediction of the behavior of modularly assembled TALER cascade and switch circuits. We also show that TALER switches employing feedback regulation exhibit improved accuracy for microRNA-based HeLa cancer cell classification versus HEK293 cells. Our TALER library is a valuable toolkit for modular engineering of synthetic circuits, enabling programmable manipulation of mammalian cells and helping elucidate design principles of coupled transcriptional and microRNA-mediated post-transcriptional regulation. äModular construction of mammalian gene circuits using TALE transcripti(epmc).pdf DeepDyve Pubget Overpricing