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10.1039/c4an01980f http://scihub22266oqcxt.onion/10.1039/c4an01980f C4331242!4331242 !25599104     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25599104 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Analyst 2015 ; 140 (5 ): 1516-22 Nephropedia Template TP {{tp|p=25599104 |t=2015. Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis |pdf=|usr=}}{{25599104 }} gab.com Text Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis JO: Analyst http://www.kidney.de/pget.php?&tw=1&pmid=25599104 #FOAvip There is a need for low cost, sensitive and chemical specific detectors for routine characterization of biomolecules. In this study, we utilize sheath-flow surface-enhanced Raman scattering (SERS) to analyze a mixture of eight biologically-active peptides separated by capillary zone electrophoresis (CZE). Analysis of the SERS electropherogram resulting from online detection resolves the characteristic Raman bands attributed to the amino acid constituents of each peptide, which enables identification. The detection limit by SERS was found to be 10(-8) M. Our results suggest that the structural information obtained from the detected vibrational modes provides complementary characterization to other chemically specific detectors like mass spectrometry and improved chemical identification over other commonly used optical-based post-chromatographic detection methods. In addition, the sheath-flow SERS detection results in band narrowing in the observed electropherogram that enables distinction of closely migrating species. The results presented here indicate that this platform can provide fast, robust, reproducible, and chemical specific detection to facilitate the characterization of peptides. Twit Text FOAVip Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis ????Analyst http://www.kidney.de/pget.php?&tw=1&pmid=25599104 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25599104 #FOAvip English Wikipedia <ref>{{Cite pmid|25599104 }}</ref> Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis #MMPMID25599104 Negri P ; Sarver SA ; Schiavone NM ; Dovichi NJ ; Schultz ZD Analyst 2015[Mar]; 140 (5 ): 1516-22 PMID25599104 show ga There is a need for low cost, sensitive and chemical specific detectors for routine characterization of biomolecules. In this study, we utilize sheath-flow surface-enhanced Raman scattering (SERS) to analyze a mixture of eight biologically-active peptides separated by capillary zone electrophoresis (CZE). Analysis of the SERS electropherogram resulting from online detection resolves the characteristic Raman bands attributed to the amino acid constituents of each peptide, which enables identification. The detection limit by SERS was found to be 10(-8) M. Our results suggest that the structural information obtained from the detected vibrational modes provides complementary characterization to other chemically specific detectors like mass spectrometry and improved chemical identification over other commonly used optical-based post-chromatographic detection methods. In addition, the sheath-flow SERS detection results in band narrowing in the observed electropherogram that enables distinction of closely migrating species. The results presented here indicate that this platform can provide fast, robust, reproducible, and chemical specific detection to facilitate the characterization of peptides. |*Online Systems [MESH] |Electrophoresis, Capillary/*methods [MESH] |Humans [MESH] |Peptide Fragments/*analysis/*chemistry [MESH] |Spectrometry, Mass, Electrospray Ionization/*methods [MESH]Online SERS detection and characterization of eight biologically-activ(epmc).pdf DeepDyve Pubget Overpricing