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10.1164/rccm.201408-1452OC http://scihub22266oqcxt.onion/10.1164/rccm.201408-1452OC C4315819!4315819!25333685     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Respir+Crit+Care+Med 2014 ; 190 (11): 1263-72 Nephropedia Template TP {{tp|p=25333685|t=2014. Relationship of DNA Methylation and Gene Expression in Idiopathic Pulmonary Fibrosis |pdf=|usr=}}{{25333685}} gab.com Text Relationship of DNA Methylation and Gene Expression in Idiopathic Pulmonary Fibrosis JO: Am J Respir Crit Care Med http://www.kidney.de/pget.php?&tw=1&pmid=25333685 #FOAvip Rationale: Idiopathic pulmonary fibrosis (IPF) is an untreatable and often fatal lung disease that is increasing in prevalence and is caused by complex interactions between genetic and environmental factors. Epigenetic mechanisms control gene expression and are likely to regulate the IPF transcriptome.Objectives: To identify methylation marks that modify gene expression in IPF lung.Methods: We assessed DNA methylation (comprehensive high-throughput arrays for relative methylation arrays [CHARM]) and gene expression (Agilent gene expression arrays) in 94 patients with IPF and 67 control subjects, and performed integrative genomic analyses to define methylation?gene expression relationships in IPF lung. We validated methylation changes by a targeted analysis (Epityper), and performed functional validation of one of the genes identified by our analysis.Measurements and Main Results: We identified 2,130 differentially methylated regions (DMRs; <5% false discovery rate), of which 738 are associated with significant changes in gene expression and enriched for expected inverse relationship between methylation and expression (P?<?2.2?×?10?16). We validated 13/15 DMRs by targeted analysis of methylation. Methylation?expression quantitative trait loci (methyl-eQTL) identified methylation marks that control cis and trans gene expression, with an enrichment for cis relationships (P?<?2.2?×?10?16). We found five trans methyl-eQTLs where a methylation change at a single DMR is associated with transcriptional changes in a substantial number of genes; four of these DMRs are near transcription factors (castor zinc finger 1 [CASZ1], FOXC1, MXD4, and ZDHHC4). We studied the in vitro effects of change in CASZ1 expression and validated its role in regulation of target genes in the methyl-eQTL.Conclusions: These results suggest that DNA methylation may be involved in the pathogenesis of IPF. Twit Text FOAVip Relationship of DNA Methylation and Gene Expression in Idiopathic Pulmonary Fibrosis ????Am J Respir Crit Care Med http://www.kidney.de/pget.php?&tw=1&pmid=25333685 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25333685 #FOAvip English Wikipedia <ref>{{Cite pmid|25333685}}</ref> Relationship of DNA Methylation and Gene Expression in Idiopathic Pulmonary Fibrosis #MMPMID25333685 Yang IV; Pedersen BS; Rabinovich E; Hennessy CE; Davidson EJ; Murphy E; Guardela BJ; Tedrow JR; Zhang Y; Singh MK; Correll M; Schwarz MI; Geraci M; Sciurba FC; Quackenbush J; Spira A; Kaminski N; Schwartz DA Am J Respir Crit Care Med 2014[Dec]; 190 (11): 1263-72 PMID25333685show ga Rationale: Idiopathic pulmonary fibrosis (IPF) is an untreatable and often fatal lung disease that is increasing in prevalence and is caused by complex interactions between genetic and environmental factors. Epigenetic mechanisms control gene expression and are likely to regulate the IPF transcriptome.Objectives: To identify methylation marks that modify gene expression in IPF lung.Methods: We assessed DNA methylation (comprehensive high-throughput arrays for relative methylation arrays [CHARM]) and gene expression (Agilent gene expression arrays) in 94 patients with IPF and 67 control subjects, and performed integrative genomic analyses to define methylation?gene expression relationships in IPF lung. We validated methylation changes by a targeted analysis (Epityper), and performed functional validation of one of the genes identified by our analysis.Measurements and Main Results: We identified 2,130 differentially methylated regions (DMRs; <5% false discovery rate), of which 738 are associated with significant changes in gene expression and enriched for expected inverse relationship between methylation and expression (P? äRelationship of DNA Methylation and Gene Expression in Idiopathic Pulm(epmc).pdf DeepDyve Pubget Overpricing