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10.1126/science.1260088 http://scihub22266oqcxt.onion/10.1126/science.1260088 C4312537!4312537!25592419     free     free     free       Science 2015 ; 347 (6221): 543-8 Nephropedia Template TP {{tp|p=25592419|t=2015. Expansion Microscopy |pdf=|usr=}}{{25592419}} gab.com Text Expansion Microscopy JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=25592419 #FOAvip In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. Here we report the discovery that, by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable super-resolution microscopy with diffraction-limited microscopes. We demonstrate ExM with effective ~70 nm lateral resolution in both cultured cells and brain tissue, performing three-color super-resolution imaging of ~107 ?m3 of the mouse hippocampus with a conventional confocal microscope. Twit Text FOAVip Expansion Microscopy ????Science http://www.kidney.de/pget.php?&tw=1&pmid=25592419 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25592419 #FOAvip English Wikipedia <ref>{{Cite pmid|25592419}}</ref> Expansion Microscopy #MMPMID25592419 Chen F; Tillberg PW; Boyden ES Science 2015[Jan]; 347 (6221): 543-8 PMID25592419show ga In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. Here we report the discovery that, by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable super-resolution microscopy with diffraction-limited microscopes. We demonstrate ExM with effective ~70 nm lateral resolution in both cultured cells and brain tissue, performing three-color super-resolution imaging of ~107 ?m3 of the mouse hippocampus with a conventional confocal microscope. äExpansion Microscopy (epmc).pdf DeepDyve Pubget Overpricing