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10.1242/dev.114025 http://scihub22266oqcxt.onion/10.1242/dev.114025 C4302916!4302916!25344074     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25344074&cmd=llinks): Failed to open stream: HTTP request failed! 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Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors |pdf=|usr=}}{{25344074}} gab.com Text Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors JO: Development http://www.kidney.de/pget.php?&tw=1&pmid=25344074 #FOAvip Various combinations of cardiogenic transcription factors, including Gata4 (G), Hand2 (H), Mef2c (M) and Tbx5 (T), can reprogram fibroblasts into induced cardiac-like myocytes (iCLMs) in vitro and in vivo. Given that optimal cardiac function relies on distinct yet functionally interconnected atrial, ventricular and pacemaker (PM) cardiomyocytes (CMs), it remains to be seen which subtypes are generated by direct reprogramming and whether this process can be harnessed to produce a specific CM of interest. Here, we employ a PM-specific Hcn4-GFP reporter mouse and a spectrum of CM subtype-specific markers to investigate the range of cellular phenotypes generated by reprogramming of primary fibroblasts. Unexpectedly, we find that a combination of four transcription factors (4F) optimized for Hcn4-GFP expression does not generate beating PM cells due to inadequate sarcomeric protein expression and organization. However, applying strict single-cell criteria to GHMT-reprogrammed cells, we observe induction of diverse cellular phenotypes, including those resembling immature forms of all three major cardiac subtypes (i.e. atrial, ventricular and pacemaker). In addition, we demonstrate that cells induced by GHMT are directly reprogrammed and do not arise from an Nxk2.5+ progenitor cell intermediate. Taken together, our results suggest a remarkable degree of plasticity inherent to GHMT reprogramming and provide a starting point for optimization of CM subtype-specific reprogramming protocols. Twit Text FOAVip Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors ????Development http://www.kidney.de/pget.php?&tw=1&pmid=25344074 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25344074 #FOAvip English Wikipedia <ref>{{Cite pmid|25344074}}</ref> Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors #MMPMID25344074 Nam YJ; Lubczyk C; Bhakta M; Zang T; Fernandez-Perez A; McAnally J; Bassel-Duby R; Olson EN; Munshi NV Development 2014[Nov]; 141 (22): 4267-78 PMID25344074show ga Various combinations of cardiogenic transcription factors, including Gata4 (G), Hand2 (H), Mef2c (M) and Tbx5 (T), can reprogram fibroblasts into induced cardiac-like myocytes (iCLMs) in vitro and in vivo. Given that optimal cardiac function relies on distinct yet functionally interconnected atrial, ventricular and pacemaker (PM) cardiomyocytes (CMs), it remains to be seen which subtypes are generated by direct reprogramming and whether this process can be harnessed to produce a specific CM of interest. Here, we employ a PM-specific Hcn4-GFP reporter mouse and a spectrum of CM subtype-specific markers to investigate the range of cellular phenotypes generated by reprogramming of primary fibroblasts. Unexpectedly, we find that a combination of four transcription factors (4F) optimized for Hcn4-GFP expression does not generate beating PM cells due to inadequate sarcomeric protein expression and organization. However, applying strict single-cell criteria to GHMT-reprogrammed cells, we observe induction of diverse cellular phenotypes, including those resembling immature forms of all three major cardiac subtypes (i.e. atrial, ventricular and pacemaker). In addition, we demonstrate that cells induced by GHMT are directly reprogrammed and do not arise from an Nxk2.5+ progenitor cell intermediate. Taken together, our results suggest a remarkable degree of plasticity inherent to GHMT reprogramming and provide a starting point for optimization of CM subtype-specific reprogramming protocols. äInduction of diverse cardiac cell types by reprogramming fibroblasts w(epmc).pdf DeepDyve Pubget Overpricing