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10.1242/dev.114488 http://scihub22266oqcxt.onion/10.1242/dev.114488 C4302896!4302896!25336740     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Development 2014 ; 141 (21): 4115-20 Nephropedia Template TP {{tp|p=25336740|t=2014. Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9 |pdf=|usr=}}{{25336740}} gab.com Text Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9 JO: Development http://www.kidney.de/pget.php?&tw=1&pmid=25336740 #FOAvip The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of the Ebf (Collier/Olf/EBF) gene in hundreds of synchronized Ciona embryos. Phenotyping of transfected embryos in the ?F0? generation revealed that endogenous Ebf function is required for specification of Islet-expressing motor ganglion neurons and atrial siphon muscles. We demonstrate that CRISPR/Cas9 is sufficiently effective and specific to generate large numbers of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening of gene function in Ciona. Twit Text FOAVip Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9 ????Development http://www.kidney.de/pget.php?&tw=1&pmid=25336740 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25336740 #FOAvip English Wikipedia <ref>{{Cite pmid|25336740}}</ref> Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9 #MMPMID25336740 Stolfi A; Gandhi S; Salek F; Christiaen L Development 2014[Nov]; 141 (21): 4115-20 PMID25336740show ga The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of the Ebf (Collier/Olf/EBF) gene in hundreds of synchronized Ciona embryos. Phenotyping of transfected embryos in the ?F0? generation revealed that endogenous Ebf function is required for specification of Islet-expressing motor ganglion neurons and atrial siphon muscles. We demonstrate that CRISPR/Cas9 is sufficiently effective and specific to generate large numbers of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening of gene function in Ciona. äTissue-specific genome editing in Ciona embryos by CRISPR/Cas9 (epmc).pdf DeepDyve Pubget Overpricing