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10.1073/pnas.1413234112 http://scihub22266oqcxt.onion/10.1073/pnas.1413234112 C4291653!4291653 !25540418     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25540418 &cmd=llinks): Failed to open stream: HTTP request failed! 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ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells |pdf=|usr=}}{{25540418 }} gab.com Text ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25540418 #FOAvip Transplantation of endothelial cells (ECs) is a promising therapeutic approach for ischemic disorders. In addition, the generation of ECs has become increasingly important for providing vascular plexus to regenerated organs, such as the liver. Although many attempts have been made to generate ECs from pluripotent stem cells and nonvascular cells, the minimum number of transcription factors that specialize in directly inducing vascular ECs remains undefined. Here, by screening 18 transcription factors that are important for both endothelial and hematopoietic development, we demonstrate that ets variant 2 (ETV2) alone directly converts primary human adult skin fibroblasts into functional vascular endothelial cells (ETVECs). In coordination with endogenous FOXC2 in fibroblasts, transduced ETV2 elicits expression of multiple key endothelial development factors, including FLI1, ERG, and TAL1, and induces expression of endothelial functional molecules, including EGFL7 and von Willebrand factor. Consequently, ETVECs exhibits EC characteristics in vitro and forms mature functional vasculature in Matrigel plugs transplanted in NOD SCID mice. Furthermore, ETVECs significantly improve blood flow recovery in a hind limb ischemic model using BALB/c-nu mice. Our study indicates that the creation of ETVECs provides further understanding of human EC development induced by ETV2. Twit Text FOAVip ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=25540418 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25540418 #FOAvip English Wikipedia <ref>{{Cite pmid|25540418 }}</ref> ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells #MMPMID25540418 Morita R ; Suzuki M ; Kasahara H ; Shimizu N ; Shichita T ; Sekiya T ; Kimura A ; Sasaki K ; Yasukawa H ; Yoshimura A Proc Natl Acad Sci U S A 2015[Jan]; 112 (1 ): 160-5 PMID25540418 show ga Transplantation of endothelial cells (ECs) is a promising therapeutic approach for ischemic disorders. In addition, the generation of ECs has become increasingly important for providing vascular plexus to regenerated organs, such as the liver. Although many attempts have been made to generate ECs from pluripotent stem cells and nonvascular cells, the minimum number of transcription factors that specialize in directly inducing vascular ECs remains undefined. Here, by screening 18 transcription factors that are important for both endothelial and hematopoietic development, we demonstrate that ets variant 2 (ETV2) alone directly converts primary human adult skin fibroblasts into functional vascular endothelial cells (ETVECs). In coordination with endogenous FOXC2 in fibroblasts, transduced ETV2 elicits expression of multiple key endothelial development factors, including FLI1, ERG, and TAL1, and induces expression of endothelial functional molecules, including EGFL7 and von Willebrand factor. Consequently, ETVECs exhibits EC characteristics in vitro and forms mature functional vasculature in Matrigel plugs transplanted in NOD SCID mice. Furthermore, ETVECs significantly improve blood flow recovery in a hind limb ischemic model using BALB/c-nu mice. Our study indicates that the creation of ETVECs provides further understanding of human EC development induced by ETV2. |Adult [MESH] |Animals [MESH] |Blood Vessels/metabolism [MESH] |Cell Proliferation [MESH] |Endothelial Cells/*cytology/*metabolism [MESH] |Fibroblasts/*cytology/*metabolism [MESH] |Forkhead Transcription Factors/metabolism [MESH] |Humans [MESH] |Mice, Inbred NOD [MESH] |Mice, SCID [MESH] |Perfusion [MESH]ETS transcription factor ETV2 directly converts human fibroblasts into(epmc).pdf DeepDyve Pubget Overpricing