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10.1111/bph.12877 http://scihub22266oqcxt.onion/10.1111/bph.12877 C4290716!4290716!25117266     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25117266&cmd=llinks): Failed to open stream: HTTP request failed! 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Structural basis for constitutive activity and agonist-induced activation of the enteroendocrine fat sensor GPR119 |pdf=|usr=}}{{25117266}} gab.com Text Structural basis for constitutive activity and agonist-induced activation of the enteroendocrine fat sensor GPR119 JO: Br J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=25117266 #FOAvip Background and Purpose: GPR119 is a G?s-coupled 7TM receptor activated by endogenous lipids such as oleoylethanolamide (OEA) and by the dietary triglyceride metabolite 2-monoacylglycerol. GPR119 stimulates enteroendocrine hormone and insulin secretion. But despite massive drug discovery efforts in the field, very little is known about the basic molecular pharmacology of GPR119. Experimental Approach: GPR119 receptor signalling was studied in transfected cells. Mutational mapping (30 mutations in 23 positions) was performed on residues required for ligand-independent and agonist-induced GPR119 activation (AR231453 and OEA). Novel Rosetta-based receptor modelling was applied, using a composite template approach with segments from different X-ray structures and fully flexible ligand docking. Key Results: The increased signalling induced by increasing the cell surface expression of GPR119 in the absence of agonist and the inhibitory effect of two synthetic inverse agonists demonstrated that GRP119 signals with a high degree of constitutive activity through the G?s pathway. The mutational maps for AR231453 and OEA were very similar and, surprisingly, also similar to the mutational map for residues affecting the constitutive signalling ? albeit with key differences. Surprisingly, almost all residues in extracellular loop-2b were important for the constitutive activity. The molecular modelling and docking demonstrated that AR231453 binds in a ?vertical? pocket in between mutational hits reaching from the centre of the receptor out to extracellular loop-2b. Conclusions and Implications: The high constitutive activity of GPR119 should be taken into account in future drug discovery efforts, which can now be guided by the detailed knowledge of the physiochemical properties of the extended ligand-binding pocket. Twit Text FOAVip Structural basis for constitutive activity and agonist-induced activation of the enteroendocrine fat sensor GPR119 ????Br J Pharmacol http://www.kidney.de/pget.php?&tw=1&pmid=25117266 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25117266 #FOAvip English Wikipedia <ref>{{Cite pmid|25117266}}</ref> Structural basis for constitutive activity and agonist-induced activation of the enteroendocrine fat sensor GPR119 #MMPMID25117266 Engelstoft MS; Norn C; Hauge M; Holliday ND; Elster L; Lehmann J; Jones RM; Frimurer TM; Schwartz TW Br J Pharmacol 2014[Dec]; 171 (24): 5774-89 PMID25117266show ga Background and Purpose: GPR119 is a G?s-coupled 7TM receptor activated by endogenous lipids such as oleoylethanolamide (OEA) and by the dietary triglyceride metabolite 2-monoacylglycerol. GPR119 stimulates enteroendocrine hormone and insulin secretion. But despite massive drug discovery efforts in the field, very little is known about the basic molecular pharmacology of GPR119. Experimental Approach: GPR119 receptor signalling was studied in transfected cells. Mutational mapping (30 mutations in 23 positions) was performed on residues required for ligand-independent and agonist-induced GPR119 activation (AR231453 and OEA). Novel Rosetta-based receptor modelling was applied, using a composite template approach with segments from different X-ray structures and fully flexible ligand docking. Key Results: The increased signalling induced by increasing the cell surface expression of GPR119 in the absence of agonist and the inhibitory effect of two synthetic inverse agonists demonstrated that GRP119 signals with a high degree of constitutive activity through the G?s pathway. The mutational maps for AR231453 and OEA were very similar and, surprisingly, also similar to the mutational map for residues affecting the constitutive signalling ? albeit with key differences. Surprisingly, almost all residues in extracellular loop-2b were important for the constitutive activity. The molecular modelling and docking demonstrated that AR231453 binds in a ?vertical? pocket in between mutational hits reaching from the centre of the receptor out to extracellular loop-2b. Conclusions and Implications: The high constitutive activity of GPR119 should be taken into account in future drug discovery efforts, which can now be guided by the detailed knowledge of the physiochemical properties of the extended ligand-binding pocket. äStructural basis for constitutive activity and agonist-induced activat(epmc).pdf DeepDyve Pubget Overpricing