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10.3390/ijms151221825 http://scihub22266oqcxt.onion/10.3390/ijms151221825 C4284680!4284680 !25431926     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25431926 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Sci 2014 ; 15 (12 ): 21825-39 Nephropedia Template TP {{tp|p=25431926 |t=2014. Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons |pdf=|usr=}}{{25431926 }} gab.com Text Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=25431926 #FOAvip Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most commonly-used technique to identify gene expression profiles. The selection of stably expressed reference genes is a prerequisite to properly evaluating gene expression. Here, the suitability of commonly-used reference genes in normalizing RT-qPCR assays of mRNA expression in cultured rat cortical neurons infected with Borna disease virus (BDV) was assessed. The expressions of eight commonly-used reference genes were comparatively analyzed in BDV-infected rat cortical neurons and non-infected control neurons mainly across 9 and 12 days post-infection. These reference genes were validated by RT-qPCR and separately ranked by four statistical algorithms: geNorm, NormFinder, BestKeeper and the comparative delta-Ct method. Then, the RankAggreg package was used to construct consensus rankings. ARBP was found to be the most stable internal control gene at Day 9, and ACTB at Day 12. As the assessment of the validity of the selected reference genes confirms the suitability of applying a combination of the two most stable references genes, combining the two most stable genes for normalization of RT-qPCR studies in BDV-infected rat cortical neurons is recommended at each time point. This study can contribute to improving BDV research by providing the means by which to obtain more reliable and accurate gene expression measurements. Twit Text FOAVip Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=25431926 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25431926 #FOAvip English Wikipedia <ref>{{Cite pmid|25431926 }}</ref> Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons #MMPMID25431926 Zhang L ; Liu S ; Zhang L ; You H ; Huang R ; Sun L ; He P ; Chen S ; Zhang H ; Xie P Int J Mol Sci 2014[Nov]; 15 (12 ): 21825-39 PMID25431926 show ga Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most commonly-used technique to identify gene expression profiles. The selection of stably expressed reference genes is a prerequisite to properly evaluating gene expression. Here, the suitability of commonly-used reference genes in normalizing RT-qPCR assays of mRNA expression in cultured rat cortical neurons infected with Borna disease virus (BDV) was assessed. The expressions of eight commonly-used reference genes were comparatively analyzed in BDV-infected rat cortical neurons and non-infected control neurons mainly across 9 and 12 days post-infection. These reference genes were validated by RT-qPCR and separately ranked by four statistical algorithms: geNorm, NormFinder, BestKeeper and the comparative delta-Ct method. Then, the RankAggreg package was used to construct consensus rankings. ARBP was found to be the most stable internal control gene at Day 9, and ACTB at Day 12. As the assessment of the validity of the selected reference genes confirms the suitability of applying a combination of the two most stable references genes, combining the two most stable genes for normalization of RT-qPCR studies in BDV-infected rat cortical neurons is recommended at each time point. This study can contribute to improving BDV research by providing the means by which to obtain more reliable and accurate gene expression measurements. |Animals [MESH] |Borna Disease/*genetics/*virology [MESH] |Borna disease virus/*physiology [MESH] |Brain-Derived Neurotrophic Factor/genetics/metabolism [MESH] |Cerebral Cortex/*pathology [MESH] |Fluorescent Antibody Technique [MESH] |Gene Expression Regulation [MESH] |Neurons/metabolism/pathology/*virology [MESH] |Rats, Sprague-Dawley [MESH] |Real-Time Polymerase Chain Reaction/*methods/*standards [MESH] |Reference Standards [MESH] |Reproducibility of Results [MESH]Real-time qPCR identifies suitable reference genes for Borna disease (epmc).pdf DeepDyve Pubget Overpricing