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10.1016/j.molimm.2014.10.021 http://scihub22266oqcxt.onion/10.1016/j.molimm.2014.10.021 C4282823!4282823 !25467797     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25467797 &cmd=llinks): Failed to open stream: HTTP request failed! 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Synergistic effect of pro-inflammatory TNF? and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis |pdf=|usr=}}{{25467797 }} gab.com Text Synergistic effect of pro-inflammatory TNF and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis JO: Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25467797 #FOAvip BACKGROUND: The pro-inflammatory cytokines, tumor necrosis factor (TNF)-?, and interleukin (IL)-17, have been implicated in the pathogenesis of liver fibrosis. In this study, we investigated the role of TNF? and IL-17 toward induction of profibrotic factor, periostin. METHODS: HepG2 cells were cultured and treated with inflammatory cytokines, TNF? and IL-17. Computational promoter sequence analysis of the periostin promoter was performed to define the putative binding sites for transcription factors. Transcription factors were analyzed by Western blot and Chromatin Immunoprecipitation. Periostin and transcription factor expression analysis was performed by RT-PCR, Western blot, and fluorescence microscopy. Type I collagen expression from fibroblast cultures was analyzed by Western blot and Sircol soluble collagen assay. RESULTS: Activation of HepG2 Cells with TNF? and IL-17 enhanced the expression of periostin (3.5 and 4.4 fold, respectively p<0.05) compared to untreated cells. However, combined treatment with both TNF? and IL-17 at similar concentration demonstrated a 13.3 fold increase in periostin (p<0.01), thus suggesting a synergistic role of these cytokines. Periostin promoter analysis and specific siRNA knock-down revealed that TNF? induces periostin through cJun, while IL-17 induced periostin via STAT-3 signaling mechanisms. Treatment of the supernatant from the cytokine activated HepG2 cells on fibroblast cultures induced enhanced expression of type I collagen (>9.1 fold, p<0.01), indicative of a direct fibrogenic effect of TNF? and IL-17. CONCLUSION: TNF? and IL-17 induced fibrogenesis through cJun and STAT-3 mediated expression of profibrotic biomarker, periostin. Therefore, periostin might serve as a novel biomarker in early diagnosis of liver fibrosis. Twit Text FOAVip Synergistic effect of pro-inflammatory TNF and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis ????Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25467797 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25467797 #FOAvip English Wikipedia <ref>{{Cite pmid|25467797 }}</ref> Synergistic effect of pro-inflammatory TNF? and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis #MMPMID25467797 Amara S ; Lopez K ; Banan B ; Brown SK ; Whalen M ; Myles E ; Ivy MT ; Johnson T ; Schey KL ; Tiriveedhi V Mol Immunol 2015[Mar]; 64 (1 ): 26-35 PMID25467797 show ga BACKGROUND: The pro-inflammatory cytokines, tumor necrosis factor (TNF)-?, and interleukin (IL)-17, have been implicated in the pathogenesis of liver fibrosis. In this study, we investigated the role of TNF? and IL-17 toward induction of profibrotic factor, periostin. METHODS: HepG2 cells were cultured and treated with inflammatory cytokines, TNF? and IL-17. Computational promoter sequence analysis of the periostin promoter was performed to define the putative binding sites for transcription factors. Transcription factors were analyzed by Western blot and Chromatin Immunoprecipitation. Periostin and transcription factor expression analysis was performed by RT-PCR, Western blot, and fluorescence microscopy. Type I collagen expression from fibroblast cultures was analyzed by Western blot and Sircol soluble collagen assay. RESULTS: Activation of HepG2 Cells with TNF? and IL-17 enhanced the expression of periostin (3.5 and 4.4 fold, respectively p<0.05) compared to untreated cells. However, combined treatment with both TNF? and IL-17 at similar concentration demonstrated a 13.3 fold increase in periostin (p<0.01), thus suggesting a synergistic role of these cytokines. Periostin promoter analysis and specific siRNA knock-down revealed that TNF? induces periostin through cJun, while IL-17 induced periostin via STAT-3 signaling mechanisms. Treatment of the supernatant from the cytokine activated HepG2 cells on fibroblast cultures induced enhanced expression of type I collagen (>9.1 fold, p<0.01), indicative of a direct fibrogenic effect of TNF? and IL-17. CONCLUSION: TNF? and IL-17 induced fibrogenesis through cJun and STAT-3 mediated expression of profibrotic biomarker, periostin. Therefore, periostin might serve as a novel biomarker in early diagnosis of liver fibrosis. |Cell Adhesion Molecules/genetics/*metabolism [MESH] |Collagen Type I/genetics/*metabolism [MESH] |DNA, Neoplasm/metabolism [MESH] |Fibroblasts/metabolism [MESH] |Hep G2 Cells [MESH] |Humans [MESH] |Inflammation Mediators/*pharmacology [MESH] |Interleukin-17/*pharmacology [MESH] |Liver Cirrhosis/*metabolism/*pathology [MESH] |Promoter Regions, Genetic/genetics [MESH] |Protein Binding [MESH] |Proto-Oncogene Proteins c-jun/metabolism [MESH] |STAT3 Transcription Factor/metabolism [MESH] |Transcription, Genetic [MESH]Synergistic effect of pro-inflammatory TNF? and IL-17 in periostin med(epmc).pdf DeepDyve Pubget Overpricing