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10.1038/nn.3892 http://scihub22266oqcxt.onion/10.1038/nn.3892 C4281300!4281300!25485758     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Neurosci 2015 ; 18 (1): 75-86 Nephropedia Template TP {{tp|p=25485758|t=2015. Cell-specific STORM superresolution imaging reveals nanoscale organization of cannabinoid signaling |pdf=|usr=}}{{25485758}} gab.com Text Cell-specific STORM superresolution imaging reveals nanoscale organization of cannabinoid signaling JO: Nat Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=25485758 #FOAvip A major challenge in neuroscience is to determine the nanoscale position and quantity of signaling molecules in a cell-type-, and subcellular compartment-specific manner. We therefore developed a novel approach combining cell-specific physiological and anatomical characterization with superresolution imaging, and studied the molecular and structural parameters shaping the physiological properties of synaptic endocannabinoid signaling in the mouse hippocampus. We found that axon terminals of perisomatically-projecting GABAergic interneurons possess increased CB1 receptor number, active-zone complexity, and receptor/effector ratio compared to dendritically-projecting interneurons, in agreement with higher efficiency of cannabinoid signaling at somatic versus dendritic synapses. Furthermore, chronic ?9-tetrahydrocannabinol administration, which reduces cannabinoid efficacy on GABA release, evoked dramatic CB1-downregulation in a dose-dependent manner. Full receptor recovery required several weeks after cessation of ?9-tetrahydrocannabinol treatment. These findings demonstrate that cell-type-specific nanoscale analysis of endogenous protein distribution is possible in brain circuits, and identify novel molecular properties controlling endocannabinoid signaling and cannabis-induced cognitive dysfunction. Twit Text FOAVip Cell-specific STORM superresolution imaging reveals nanoscale organization of cannabinoid signaling ????Nat Neurosci http://www.kidney.de/pget.php?&tw=1&pmid=25485758 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25485758 #FOAvip English Wikipedia <ref>{{Cite pmid|25485758}}</ref> Cell-specific STORM superresolution imaging reveals nanoscale organization of cannabinoid signaling #MMPMID25485758 Dudok B; Barna L; Ledri M; Szabó SI; Szabadits E; Pintér B; Woodhams SG; Henstridge CM; Balla GY; Nyilas R; Varga C; Lee SH; Matolcsi M; Cervenak J; Kacskovics I; Watanabe M; Sagheddu C; Melis M; Pistis M; Soltesz I; Katona I Nat Neurosci 2015[Jan]; 18 (1): 75-86 PMID25485758show ga A major challenge in neuroscience is to determine the nanoscale position and quantity of signaling molecules in a cell-type-, and subcellular compartment-specific manner. We therefore developed a novel approach combining cell-specific physiological and anatomical characterization with superresolution imaging, and studied the molecular and structural parameters shaping the physiological properties of synaptic endocannabinoid signaling in the mouse hippocampus. We found that axon terminals of perisomatically-projecting GABAergic interneurons possess increased CB1 receptor number, active-zone complexity, and receptor/effector ratio compared to dendritically-projecting interneurons, in agreement with higher efficiency of cannabinoid signaling at somatic versus dendritic synapses. Furthermore, chronic ?9-tetrahydrocannabinol administration, which reduces cannabinoid efficacy on GABA release, evoked dramatic CB1-downregulation in a dose-dependent manner. Full receptor recovery required several weeks after cessation of ?9-tetrahydrocannabinol treatment. These findings demonstrate that cell-type-specific nanoscale analysis of endogenous protein distribution is possible in brain circuits, and identify novel molecular properties controlling endocannabinoid signaling and cannabis-induced cognitive dysfunction. äCell-specific STORM superresolution imaging reveals nanoscale organiza(epmc).pdf DeepDyve Pubget Overpricing