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10.1002/cbic.201402433 http://scihub22266oqcxt.onion/10.1002/cbic.201402433 C4278937!4278937!25403986     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chembiochem 2014 ; 15 (18): 2667-73 Nephropedia Template TP {{tp|p=25403986|t=2014. CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes |pdf=|usr=}}{{25403986}} gab.com Text CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes JO: Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=25403986 #FOAvip The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for their detection. Here, we report the development of a histological method, dubbed CHoMP?Chemoenzymatic Histology of Membrane Polysaccharides?for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analog equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, suggestion a potential application of this technique in early cancer diagnosis. Twit Text FOAVip CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes ????Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=25403986 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25403986 #FOAvip English Wikipedia <ref>{{Cite pmid|25403986}}</ref> CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes #MMPMID25403986 Rouhanifard SH; López-Aguilar A; Wu P Chembiochem 2014[Dec]; 15 (18): 2667-73 PMID25403986show ga The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for their detection. Here, we report the development of a histological method, dubbed CHoMP?Chemoenzymatic Histology of Membrane Polysaccharides?for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analog equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, suggestion a potential application of this technique in early cancer diagnosis. äCHoMP: A Chemoenzymatic Histology Method Using Clickable Probes (epmc).pdf DeepDyve Pubget Overpricing