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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Appl+Environ+Microbiol
2015 ; 81
(2
): 569-77
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Genomic diversity and virulence profiles of historical Escherichia coli O157
strains isolated from clinical and environmental sources
#MMPMID25381234
Rump LV
; Gonzalez-Escalona N
; Ju W
; Wang F
; Cao G
; Meng S
; Meng J
Appl Environ Microbiol
2015[Jan]; 81
(2
): 569-77
PMID25381234
show ga
Escherichia coli O157:H7 is, to date, the major E. coli serotype causing
food-borne human disease worldwide. Strains of O157 with other H antigens also
have been recovered. We analyzed a collection of historic O157 strains (n = 400)
isolated in the late 1980s to early 1990s in the United States. Strains were
predominantly serotype O157:H7 (55%), and various O157:non-H7 (41%) serotypes
were not previously reported regarding their pathogenic potential. Although
lacking Shiga toxin (stx) and eae genes, serotypes O157:H1, O157:H2, O157:H11,
O157:H42, and O157:H43 carried several virulence factors (iha, terD, and hlyA)
also found in virulent serotype E. coli O157:H7. Pulsed-field gel electrophoresis
(PFGE) showed the O157 serogroup was diverse, with strains with the same H type
clustering together closely. Among non-H7 isolates, serotype O157:H43 was highly
prevalent (65%) and carried important enterohemorrhagic E. coli (EHEC) virulence
markers (iha, terD, hlyA, and espP). Isolates from two particular H types, H2 and
H11, among the most commonly found non-O157 EHEC serotypes (O26:H11, O111:H11,
O103:H2/H11, and O45:H2), unexpectedly clustered more closely with O157:H7 than
other H types and carried several virulence genes. This suggests an early
divergence of the O157 serogroup to clades with different pathogenic potentials.
The appearance of important EHEC virulence markers in closely related H types
suggests their virulence potential and suggests further monitoring of those
serotypes not implicated in severe illness thus far.