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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Cell+Biol
2014 ; 207
(6
): 683-93
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KIF4A and PP2A-B56 form a spatially restricted feedback loop opposing Aurora B at
the anaphase central spindle
#MMPMID25512391
Bastos RN
; Cundell MJ
; Barr FA
J Cell Biol
2014[Dec]; 207
(6
): 683-93
PMID25512391
show ga
The mitotic kinase Aurora B is concentrated at the anaphase central spindle by
the kinesin MKlp2 during mitotic exit and cytokinesis. This pool of Aurora B
phosphorylates substrates including the kinesin KIF4A to regulate central spindle
length. In this paper, we identify a counteracting system in which PP2A-B56? and
-?, but not PP2A-B56?, -?, and -?, are maintained at the central spindle by
KIF4A. Biochemical assays show that PP2A-B56? can dephosphorylate the T799 Aurora
B site on KIF4A and thereby counteract the Aurora B- and microtubule-stimulated
ATPase activity of KIF4A. In agreement with these observations, combined
silencing of PP2A-B56? and -? resulted in increased phosphorylation of KIF4A T799
and decreased central spindle growth in anaphase B. Furthermore, reduced turnover
of regulatory phosphorylation on another Aurora B substrate MKlp1 was observed,
suggesting that PP2A-B56? and -? play a general role opposing Aurora B at the
central spindle. KIF4A and PP2A-B56? and -? therefore create a spatially
restricted negative feedback loop counteracting Aurora B in anaphase.