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10.1073/pnas.1414184111

http://scihub22266oqcxt.onion/10.1073/pnas.1414184111
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C4273389!4273389!25453075
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suck abstract from ncbi


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pmid25453075      Proc+Natl+Acad+Sci+U+S+A 2014 ; 111 (50): 17845-50
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  • Power transduction of actin filaments ratcheting in vitro against a load #MMPMID25453075
  • Démoulin D; Carlier MF; Bibette J; Baudry J
  • Proc Natl Acad Sci U S A 2014[Dec]; 111 (50): 17845-50 PMID25453075show ga
  • How the continuous polymerization of actin filaments pushes the cell membrane forward in migrating cells is an intriguing issue that has yet to be fully understood. Molecular mechanisms were proposed, but testing them experimentally is a challenge, due to hardly accessible length scales and to the influence of regulatory proteins constantly changing the mechanical properties of polymerizing filaments. We present an experimental setup designed to minimize the number of parameters influencing filament growth to focus on the mechanism of force generation. We demonstrate that it is consistent with the Brownian ratchet model. Furthermore, we define a criterion for cell migration efficiency and quantify the number of filaments that need to push the membrane to maximize this efficiency.
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