Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet
might drive rapid cytokine production
#MMPMID25378399
Yu SF
; Zhang YN
; Yang BY
; Wu CY
J Biol Chem
2014[Dec]; 289
(51
): 35561-9
PMID25378399
show ga
We found that after stimulation for a few hours, memory but not naive CD4(+) T
cells produced a large amount of IFN-?; however, the mechanism of rapid response
of memory CD4(+) T cells remains undefined. We compared the expression of
transcription factors in resting or activated naive and memory CD4(+) T cells and
found that T-bet, but not pSTAT-1 or pSTAT-4, was highly expressed in resting
memory CD4(+) T cells and that phenotypic characteristics of T-bet(+)CD4(+) T
cells were CD45RA(low)CD62L(low) CCR7(low). After short-term stimulation,
purified memory CD4(+) T cells rapidly produced effector cytokines that were
closely associated with the pre-existence of T-bet. By contrast, resting naive
CD4(+) T cells did not express T-bet, and they produced cytokines only after
sustained stimulation. Our further studies indicated that T-bet was expressed in
the nuclei of resting memory CD4(+) T cells, which might have important
implications for rapid IFN-? production. Our results indicate that the
pre-existence and nuclear mobilization of T-bet in resting memory CD4(+) T cells
might be a possible transcriptional mechanism for rapid production of cytokines
by human memory CD4(+) T cells.
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