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10.1016/j.bpj.2014.09.050 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.09.050 C4269786!4269786!25517157     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+J 2014 ; 107 (12): 2923-31 Nephropedia Template TP {{tp|p=25517157|t=2014. Direct Observation of Subunit Exchange along Mature Vimentin Intermediate Filaments |pdf=|usr=}}{{25517157}} gab.com Text Direct Observation of Subunit Exchange along Mature Vimentin Intermediate Filaments JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25517157 #FOAvip Actin filaments, microtubules, and intermediate filaments (IFs) are central elements of the metazoan cytoskeleton. At the molecular level, the assembly mechanism for actin filaments and microtubules is fundamentally different from that of IFs. The former two types of filaments assemble from globular proteins. By contrast, IFs assemble from tetrameric complexes of extended, half-staggered, and antiparallel oriented coiled-coils. These tetramers laterally associate into unit-length filaments; subsequent longitudinal annealing of unit-length filaments yields mature IFs. In vitro, IFs form open structures without a fixed number of tetramers per cross-section along the filament. Therefore, a central question for the structural biology of IFs is whether individual subunits can dissociate from assembled filaments and rebind at other sites. Using the fluorescently labeled IF-protein vimentin for assembly, we directly observe and quantitatively determine subunit exchange events between filaments as well as with soluble vimentin pools. Thereby we demonstrate that the cross-sectional polymorphism of donor and acceptor filaments plays an important role. We propose that in segments of donor filaments with more than the standard 32 molecules per cross-section, subunits are not as tightly bound and are predisposed to be released from the filament. Twit Text FOAVip Direct Observation of Subunit Exchange along Mature Vimentin Intermediate Filaments ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25517157 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25517157 #FOAvip English Wikipedia <ref>{{Cite pmid|25517157}}</ref> Direct Observation of Subunit Exchange along Mature Vimentin Intermediate Filaments #MMPMID25517157 Nöding B; Herrmann H; Köster S Biophys J 2014[Dec]; 107 (12): 2923-31 PMID25517157show ga Actin filaments, microtubules, and intermediate filaments (IFs) are central elements of the metazoan cytoskeleton. At the molecular level, the assembly mechanism for actin filaments and microtubules is fundamentally different from that of IFs. The former two types of filaments assemble from globular proteins. By contrast, IFs assemble from tetrameric complexes of extended, half-staggered, and antiparallel oriented coiled-coils. These tetramers laterally associate into unit-length filaments; subsequent longitudinal annealing of unit-length filaments yields mature IFs. In vitro, IFs form open structures without a fixed number of tetramers per cross-section along the filament. Therefore, a central question for the structural biology of IFs is whether individual subunits can dissociate from assembled filaments and rebind at other sites. Using the fluorescently labeled IF-protein vimentin for assembly, we directly observe and quantitatively determine subunit exchange events between filaments as well as with soluble vimentin pools. Thereby we demonstrate that the cross-sectional polymorphism of donor and acceptor filaments plays an important role. We propose that in segments of donor filaments with more than the standard 32 molecules per cross-section, subunits are not as tightly bound and are predisposed to be released from the filament. äDirect Observation of Subunit Exchange along Mature Vimentin Intermedi(epmc).pdf DeepDyve Pubget Overpricing