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10.1016/j.jim.2014.10.010 http://scihub22266oqcxt.onion/10.1016/j.jim.2014.10.010 C4269324!4269324!25450003     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Methods 2014 ; 415 (ä): 1-5 Nephropedia Template TP {{tp|p=25450003|t=2014. CyTOF supports efficient detection of immune cell subsets from small samples |pdf=|usr=}}{{25450003}} gab.com Text CyTOF supports efficient detection of immune cell subsets from small samples JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=25450003 #FOAvip Analysis of immune cell states is paramount to our understanding of the pathogenesis of a broad range of human diseases. Immunologists rely on fluorescence cytometry for cellular analysis, and while detection of 8 markers is now well established, the overlap of fluorescent signals limits efficiency. Mass cytometry or CyTOF (Cytometry by Time-Of-Flight) is a new technology for multiparameter single cell analysis that overcomes many limitations of fluorescence-based flow cytometry and can routinely detect as many as 40 markers per sample. This technology provides tremendous detail for cellular analysis of multiple cell populations simultaneously and is a powerful technique for translational investigations. Here we present reproducible detection of immune cell subsets starting with as few as 10,000 cells. Our study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research. Twit Text FOAVip CyTOF supports efficient detection of immune cell subsets from small samples ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=25450003 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25450003 #FOAvip English Wikipedia <ref>{{Cite pmid|25450003}}</ref> CyTOF supports efficient detection of immune cell subsets from small samples #MMPMID25450003 Yao Y; Liu R; Shin MS; Trentalange M; Allore H; Nassar A; Kang I; Pober J; Montgomery RR J Immunol Methods 2014[Dec]; 415 (ä): 1-5 PMID25450003show ga Analysis of immune cell states is paramount to our understanding of the pathogenesis of a broad range of human diseases. Immunologists rely on fluorescence cytometry for cellular analysis, and while detection of 8 markers is now well established, the overlap of fluorescent signals limits efficiency. Mass cytometry or CyTOF (Cytometry by Time-Of-Flight) is a new technology for multiparameter single cell analysis that overcomes many limitations of fluorescence-based flow cytometry and can routinely detect as many as 40 markers per sample. This technology provides tremendous detail for cellular analysis of multiple cell populations simultaneously and is a powerful technique for translational investigations. Here we present reproducible detection of immune cell subsets starting with as few as 10,000 cells. Our study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research. äCyTOF supports efficient detection of immune cell subsets from small s(epmc).pdf DeepDyve Pubget Overpricing