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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Invest+Dermatol
2015 ; 135
(1
): 84-93
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The skin immune atlas: three-dimensional analysis of cutaneous leukocyte subsets
by multiphoton microscopy
#MMPMID25007044
Tong PL
; Roediger B
; Kolesnikoff N
; Biro M
; Tay SS
; Jain R
; Shaw LE
; Grimbaldeston MA
; Weninger W
J Invest Dermatol
2015[Jan]; 135
(1
): 84-93
PMID25007044
show ga
Site-specific differences in skin response to pathogens and in the course of
cutaneous inflammatory diseases are well appreciated. The composition and
localization of cutaneous leukocytes has been studied extensively using histology
and flow cytometry. However, the precise three-dimensional (3D) distribution of
distinct immune cell subsets within skin at different body sites requires
visualization of intact living skin. We used intravital multiphoton microscopy in
transgenic reporter mice in combination with quantitative flow cytometry to
generate a 3D immune cell atlas of mouse skin. The 3D location of innate and
adaptive immune cells and site-specific differences in the densities of
macrophages, T cells, and mast cells at four defined sites (ear, back, footpad,
and tail) is presented. The combinatorial approach further demonstrates an as yet
unreported age-dependent expansion of dermal gamma-delta T cells. Localization of
dermal immune cells relative to anatomical structures was also determined.
Although dendritic cells were dispersed homogeneously within the dermis, mast
cells preferentially localized to the perivascular space. Finally, we show the
functional relevance of site-specific mast cell disparities using the passive
cutaneous anaphylaxis model. These approaches are applicable to assessing immune
cell variations and potential functional consequences in the setting of
infection, as well as the pathogenesis of inflammatory skin conditions.
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