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10.1089/lrb.2014.0012 http://scihub22266oqcxt.onion/10.1089/lrb.2014.0012 C4267131!4267131!25412238     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lymphat+Res+Biol 2014 ; 12 (4): 238-50 Nephropedia Template TP {{tp|p=25412238|t=2014. Radiation-Induced Impairment in Lung Lymphatic Vasculature |pdf=|usr=}}{{25412238}} gab.com Text Radiation-Induced Impairment in Lung Lymphatic Vasculature JO: Lymphat Res Biol http://www.kidney.de/pget.php?&tw=1&pmid=25412238 #FOAvip Background: The lymphatic vasculature has been shown to play important roles in lung injury and repair, particularly in lung fibrosis. The effects of ionizing radiation on lung lymphatic vasculature have not been previously reported.Methods and Results: C57Bl/6 mice were immobilized in a lead shield exposing only the thoracic cavity, and were irradiated with a single dose of 14 Gy. Animals were sacrificed and lungs collected at different time points (1, 4, 8, and 16 weeks) following radiation. To identify lymphatic vessels in lung tissue sections, we used antibodies that are specific for lymphatic vessel endothelial receptor 1 (LYVE-1), a marker of lymphatic endothelial cells (LEC). To evaluate LEC cell death and oxidative damage, lung tissue sections were stained for LYVE-1 and with TUNEL staining, or 8-oxo-dG respectively. Images were imported into ImageJ v1.36b and analyzed. Compared to a non-irradiated control group, we observed a durable and progressive decrease in the density, perimeter, and area of lymphatic vessels over the study period. The decline in the density of lymphatic vessels was observed in both subpleural and interstitial lymphatics. Histopathologically discernible pulmonary fibrosis was not apparent until 16 weeks after irradiation. Furthermore, there was significantly increased LEC apoptosis and oxidative damage at one week post-irradiation that persisted at 16 weeks.Conclusions: There is impairment of lymphatic vasculature after a single dose of ionizing radiation that precedes architectural distortion and fibrosis, suggesting important roles for the lymphatic circulation in the pathogenesis of the radiation-induced lung injury. Twit Text FOAVip Radiation-Induced Impairment in Lung Lymphatic Vasculature ????Lymphat Res Biol http://www.kidney.de/pget.php?&tw=1&pmid=25412238 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25412238 #FOAvip English Wikipedia <ref>{{Cite pmid|25412238}}</ref> Radiation-Induced Impairment in Lung Lymphatic Vasculature #MMPMID25412238 Cui Y; Wilder J; Rietz C; Gigliotti A; Tang X; Shi Y; Guilmette R; Wang H; George G; de Magaldi EN; Chu SG; Doyle-Eisele M; McDonald JD; Rosas IO; El-Chemaly S Lymphat Res Biol 2014[Dec]; 12 (4): 238-50 PMID25412238show ga Background: The lymphatic vasculature has been shown to play important roles in lung injury and repair, particularly in lung fibrosis. The effects of ionizing radiation on lung lymphatic vasculature have not been previously reported.Methods and Results: C57Bl/6 mice were immobilized in a lead shield exposing only the thoracic cavity, and were irradiated with a single dose of 14 Gy. Animals were sacrificed and lungs collected at different time points (1, 4, 8, and 16 weeks) following radiation. To identify lymphatic vessels in lung tissue sections, we used antibodies that are specific for lymphatic vessel endothelial receptor 1 (LYVE-1), a marker of lymphatic endothelial cells (LEC). To evaluate LEC cell death and oxidative damage, lung tissue sections were stained for LYVE-1 and with TUNEL staining, or 8-oxo-dG respectively. Images were imported into ImageJ v1.36b and analyzed. Compared to a non-irradiated control group, we observed a durable and progressive decrease in the density, perimeter, and area of lymphatic vessels over the study period. The decline in the density of lymphatic vessels was observed in both subpleural and interstitial lymphatics. Histopathologically discernible pulmonary fibrosis was not apparent until 16 weeks after irradiation. Furthermore, there was significantly increased LEC apoptosis and oxidative damage at one week post-irradiation that persisted at 16 weeks.Conclusions: There is impairment of lymphatic vasculature after a single dose of ionizing radiation that precedes architectural distortion and fibrosis, suggesting important roles for the lymphatic circulation in the pathogenesis of the radiation-induced lung injury. äRadiation-Induced Impairment in Lung Lymphatic Vasculature (epmc).pdf DeepDyve Pubget Overpricing