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10.1038/nsmb.2914 http://scihub22266oqcxt.onion/10.1038/nsmb.2914 C4259101!4259101!25383669     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2014 ; 21 (12): 1028-34 Nephropedia Template TP {{tp|p=25383669|t=2014. Reconstitution of active human core Mediator complex reveals a pivotal role of the MED14 subunit |pdf=|usr=}}{{25383669}} gab.com Text Reconstitution of active human core Mediator complex reveals a pivotal role of the MED14 subunit JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25383669 #FOAvip The evolutionarily conserved Mediator complex is a critical coactivator for RNA polymerase II (Pol II)-mediated transcription. Here, we report the reconstitution of a functional 15-subunit human core Mediator complex and its characterization by functional assays and chemical cross-linking coupled to mass spectrometry (CX-MS). Whereas the reconstituted head and middle modules can stably associate, only with incorporation of MED14 into the bi-modular complex does it acquire basal and coactivator functions. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. Altogether, our analyses identify MED14 as both an architectural and a functional backbone of the Mediator complex. We further establish a conditional requirement for metazoan-specific MED26 that becomes evident in the presence of heterologous nuclear factors. This general approach paves the way for systematically dissecting the multiple layers of functionalities associated with the Mediator complex. Twit Text FOAVip Reconstitution of active human core Mediator complex reveals a pivotal role of the MED14 subunit ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25383669 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25383669 #FOAvip English Wikipedia <ref>{{Cite pmid|25383669}}</ref> Reconstitution of active human core Mediator complex reveals a pivotal role of the MED14 subunit #MMPMID25383669 Cevher MA; Shi Y; Li D; Chait BT; Malik S; Roeder RG Nat Struct Mol Biol 2014[Dec]; 21 (12): 1028-34 PMID25383669show ga The evolutionarily conserved Mediator complex is a critical coactivator for RNA polymerase II (Pol II)-mediated transcription. Here, we report the reconstitution of a functional 15-subunit human core Mediator complex and its characterization by functional assays and chemical cross-linking coupled to mass spectrometry (CX-MS). Whereas the reconstituted head and middle modules can stably associate, only with incorporation of MED14 into the bi-modular complex does it acquire basal and coactivator functions. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II. Altogether, our analyses identify MED14 as both an architectural and a functional backbone of the Mediator complex. We further establish a conditional requirement for metazoan-specific MED26 that becomes evident in the presence of heterologous nuclear factors. This general approach paves the way for systematically dissecting the multiple layers of functionalities associated with the Mediator complex. äReconstitution of active human core Mediator complex reveals a pivotal(epmc).pdf DeepDyve Pubget Overpricing