Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1074/mcp.M114.042630 http://scihub22266oqcxt.onion/10.1074/mcp.M114.042630 C4256503!4256503!25253489     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25253489&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Proteomics 2014 ; 13 (12): 3533-43 Nephropedia Template TP {{tp|p=25253489|t=2014. A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein?Protein Interactions in Living Cells* |pdf=|usr=}}{{25253489}} gab.com Text A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein Protein Interactions in Living Cells* JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25253489 #FOAvip Protein?protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving the physical contacts between proteins as they occur in cells is critical to uncovering the molecular details underlying various cellular activities. To advance the study of PPIs in living cells, we have developed a new in vivo cross-linking mass spectrometry platform that couples a novel membrane-permeable, enrichable, and MS-cleavable cross-linker with multistage tandem mass spectrometry. This strategy permits the effective capture, enrichment, and identification of in vivo cross-linked products from mammalian cells and thus enables the determination of protein interaction interfaces. The utility of the developed method has been demonstrated by profiling PPIs in mammalian cells at the proteome scale and the targeted protein complex level. Our work represents a general approach for studying in vivo PPIs and provides a solid foundation for future studies toward the complete mapping of PPI networks in living systems. Twit Text FOAVip A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein Protein Interactions in Living Cells* ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25253489 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25253489 #FOAvip English Wikipedia <ref>{{Cite pmid|25253489}}</ref> A New in Vivo Cross-linking Mass Spectrometry Platform to Define Protein?Protein Interactions in Living Cells* #MMPMID25253489 Kaake RM; Wang X; Burke A; Yu C; Kandur W; Yang Y; Novtisky EJ; Second T; Duan J; Kao A; Guan S; Vellucci D; Rychnovsky SD; Huang L Mol Cell Proteomics 2014[Dec]; 13 (12): 3533-43 PMID25253489show ga Protein?protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving the physical contacts between proteins as they occur in cells is critical to uncovering the molecular details underlying various cellular activities. To advance the study of PPIs in living cells, we have developed a new in vivo cross-linking mass spectrometry platform that couples a novel membrane-permeable, enrichable, and MS-cleavable cross-linker with multistage tandem mass spectrometry. This strategy permits the effective capture, enrichment, and identification of in vivo cross-linked products from mammalian cells and thus enables the determination of protein interaction interfaces. The utility of the developed method has been demonstrated by profiling PPIs in mammalian cells at the proteome scale and the targeted protein complex level. Our work represents a general approach for studying in vivo PPIs and provides a solid foundation for future studies toward the complete mapping of PPI networks in living systems. äA New in Vivo Cross-linking Mass Spectrometry Platform to Define Prote(epmc).pdf DeepDyve Pubget Overpricing