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10.1074/mcp.M114.040121 http://scihub22266oqcxt.onion/10.1074/mcp.M114.040121 C4256494!4256494 !25205225     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25205225 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell+Proteomics 2014 ; 13 (12 ): 3421-34 Nephropedia Template TP {{tp|p=25205225 |t=2014. Label-free quantitative urinary proteomics identifies the arginase pathway as a new player in congenital obstructive nephropathy |pdf=|usr=}}{{25205225 }} gab.com Text Label-free quantitative urinary proteomics identifies the arginase pathway as a new player in congenital obstructive nephropathy JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25205225 #FOAvip Obstructive nephropathy is a frequently encountered situation in newborns. In previous studies, the urinary peptidome has been analyzed for the identification of clinically useful biomarkers of obstructive nephropathy. However, the urinary proteome has not been explored yet and should allow additional insight into the pathophysiology of the disease. We have analyzed the urinary proteome of newborns (n = 5/group) with obstructive nephropathy using label free quantitative nanoLC-MS/MS allowing the identification and quantification of 970 urinary proteins. We next focused on proteins exclusively regulated in severe obstructive nephropathy and identified Arginase 1 as a potential candidate molecule involved in the development of obstructive nephropathy, located at the crossroad of pro- and antifibrotic pathways. The reduced urinary abundance of Arginase 1 in obstructive nephropathy was verified in independent clinical samples using both Western blot and MRM analysis. These data were confirmed in situ in kidneys obtained from a mouse obstructive nephropathy model. In addition, we also observed increased expression of Arginase 2 and increased total arginase activity in obstructed mouse kidneys. mRNA expression analysis of the related arginase pathways indicated that the pro-fibrotic arginase-related pathway is activated during obstructive nephropathy. Taken together we have identified a new actor in the development of obstructive nephropathy in newborns using quantitative urinary proteomics and shown its involvement in an in vivo model of disease. The present study demonstrates the relevance of such a quantitative urinary proteomics approach with clinical samples for a better understanding of the pathophysiology and for the discovery of potential therapeutic targets. Twit Text FOAVip Label-free quantitative urinary proteomics identifies the arginase pathway as a new player in congenital obstructive nephropathy ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=25205225 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25205225 #FOAvip English Wikipedia <ref>{{Cite pmid|25205225 }}</ref> Label-free quantitative urinary proteomics identifies the arginase pathway as a new player in congenital obstructive nephropathy #MMPMID25205225 Lacroix C ; Caubet C ; Gonzalez-de-Peredo A ; Breuil B ; Bouyssié D ; Stella A ; Garrigues L ; Le Gall C ; Raevel A ; Massoubre A ; Klein J ; Decramer S ; Sabourdy F ; Bandin F ; Burlet-Schiltz O ; Monsarrat B ; Schanstra JP ; Bascands JL Mol Cell Proteomics 2014[Dec]; 13 (12 ): 3421-34 PMID25205225 show ga Obstructive nephropathy is a frequently encountered situation in newborns. In previous studies, the urinary peptidome has been analyzed for the identification of clinically useful biomarkers of obstructive nephropathy. However, the urinary proteome has not been explored yet and should allow additional insight into the pathophysiology of the disease. We have analyzed the urinary proteome of newborns (n = 5/group) with obstructive nephropathy using label free quantitative nanoLC-MS/MS allowing the identification and quantification of 970 urinary proteins. We next focused on proteins exclusively regulated in severe obstructive nephropathy and identified Arginase 1 as a potential candidate molecule involved in the development of obstructive nephropathy, located at the crossroad of pro- and antifibrotic pathways. The reduced urinary abundance of Arginase 1 in obstructive nephropathy was verified in independent clinical samples using both Western blot and MRM analysis. These data were confirmed in situ in kidneys obtained from a mouse obstructive nephropathy model. In addition, we also observed increased expression of Arginase 2 and increased total arginase activity in obstructed mouse kidneys. mRNA expression analysis of the related arginase pathways indicated that the pro-fibrotic arginase-related pathway is activated during obstructive nephropathy. Taken together we have identified a new actor in the development of obstructive nephropathy in newborns using quantitative urinary proteomics and shown its involvement in an in vivo model of disease. The present study demonstrates the relevance of such a quantitative urinary proteomics approach with clinical samples for a better understanding of the pathophysiology and for the discovery of potential therapeutic targets. |Animals [MESH] |Arginase/genetics/*urine [MESH] |Disease Models, Animal [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Hydronephrosis/congenital/pathology/*urine [MESH] |Infant [MESH] |Infant, Newborn [MESH] |Kidney/*metabolism/pathology [MESH] |Male [MESH] |Mice, Inbred C57BL [MESH] |Proteome/genetics/*metabolism [MESH] |Proteomics/methods [MESH] |Renal Insufficiency/congenital/pathology/*urine [MESH]Label-free quantitative urinary proteomics identifies the arginase pat(epmc).pdf DeepDyve Pubget Overpricing