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10.1016/j.bpj.2014.09.048 http://scihub22266oqcxt.onion/10.1016/j.bpj.2014.09.048 C4255200!4255200 !25468343     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25468343 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biophys+J 2014 ; 107 (11 ): 2639-51 Nephropedia Template TP {{tp|p=25468343 |t=2014. Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex |pdf=|usr=}}{{25468343 }} gab.com Text Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex JO: Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25468343 #FOAvip We investigated the association of signaling proteins with epidermal growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered array of micron-sized features on silicon surfaces. Using NIH-3T3 cells stably expressing EGFR, we observe concentration of fluorescently labeled receptors and stimulated tyrosine phosphorylation that are spatially confined to the regions of immobilized EGF and quantified by cross-correlation analysis. We observe recruitment of phosphorylated paxillin to activated EGFR at these patterned features, as well as ?1-containing integrins that preferentially localize to more peripheral EGF features, as quantified by radial fluorescence analysis. In addition, we detect recruitment of EGFP-Ras, MEK, and phosphorylated Erk to patterned EGF in a process that depends on F-actin and phosphoinositides. These studies reveal and quantify the coformation of multiprotein EGFR signaling complexes at the plasma membrane in response to micropatterned growth factors. Twit Text FOAVip Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex ????Biophys J http://www.kidney.de/pget.php?&tw=1&pmid=25468343 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25468343 #FOAvip English Wikipedia <ref>{{Cite pmid|25468343 }}</ref> Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex #MMPMID25468343 Singhai A ; Wakefield DL ; Bryant KL ; Hammes SR ; Holowka D ; Baird B Biophys J 2014[Dec]; 107 (11 ): 2639-51 PMID25468343 show ga We investigated the association of signaling proteins with epidermal growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered array of micron-sized features on silicon surfaces. Using NIH-3T3 cells stably expressing EGFR, we observe concentration of fluorescently labeled receptors and stimulated tyrosine phosphorylation that are spatially confined to the regions of immobilized EGF and quantified by cross-correlation analysis. We observe recruitment of phosphorylated paxillin to activated EGFR at these patterned features, as well as ?1-containing integrins that preferentially localize to more peripheral EGF features, as quantified by radial fluorescence analysis. In addition, we detect recruitment of EGFP-Ras, MEK, and phosphorylated Erk to patterned EGF in a process that depends on F-actin and phosphoinositides. These studies reveal and quantify the coformation of multiprotein EGFR signaling complexes at the plasma membrane in response to micropatterned growth factors. |*MAP Kinase Signaling System/drug effects [MESH] |Actin Cytoskeleton/drug effects/metabolism [MESH] |Actins/*metabolism [MESH] |Animals [MESH] |Cell Membrane/drug effects/metabolism [MESH] |Dynamin II/metabolism [MESH] |Epidermal Growth Factor/pharmacology [MESH] |ErbB Receptors/*metabolism [MESH] |Integrin beta1/metabolism [MESH] |Ligands [MESH] |Mice [MESH] |NIH 3T3 Cells [MESH] |Paxillin/metabolism [MESH] |Phosphatidylinositol 4,5-Diphosphate/metabolism [MESH] |Phosphorylation/drug effects [MESH] |Phosphotyrosine/metabolism [MESH] |Polymerization/drug effects [MESH]Spatially defined EGF receptor activation reveals an F-actin-dependent(epmc).pdf DeepDyve Pubget Overpricing