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10.1016/j.str.2014.09.017 http://scihub22266oqcxt.onion/10.1016/j.str.2014.09.017 C4255137!4255137!25456413     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Structure 2014 ; 22 (12): 1875-82 Nephropedia Template TP {{tp|p=25456413|t=2014. Controlled Bacterial Lysis for Electron Tomography of Native Cell Membranes |pdf=|usr=}}{{25456413}} gab.com Text Controlled Bacterial Lysis for Electron Tomography of Native Cell Membranes JO: Structure http://www.kidney.de/pget.php?&tw=1&pmid=25456413 #FOAvip Cryo-electron tomography (cryoET) has become a powerful tool for direct visualization of 3D structures of native biological specimens at molecular resolution, but its application is limited to thin specimens (<300 nm). Recently, vitreous sectioning and cryo-FIB milling technologies were developed to physically reduce the specimen thickness; however, cryoET analysis of membrane protein complexes within native cell membranes remains a great challenge. Here, we use phage ?X174 lysis gene E to rapidly produce native, intact, bacterial cell membranes for high resolution cryoET. We characterized E gene-induced cell lysis using FIB/SEM and cryoEM and show that the bacteria cytoplasm was largely depleted through spot lesion, producing ghosts with the cell membranes intact. We further demonstrate the utility of E-gene-induced lysis for cryoET using the bacterial chemotaxis receptor signaling complex array. The described method should have a broad application for structural and functional studies of native, intact cell membranes and membrane protein complexes. Twit Text FOAVip Controlled Bacterial Lysis for Electron Tomography of Native Cell Membranes ????Structure http://www.kidney.de/pget.php?&tw=1&pmid=25456413 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25456413 #FOAvip English Wikipedia <ref>{{Cite pmid|25456413}}</ref> Controlled Bacterial Lysis for Electron Tomography of Native Cell Membranes #MMPMID25456413 Fu X; Himes B; Ke D; Rice WJ; Ning J; Zhang P Structure 2014[Dec]; 22 (12): 1875-82 PMID25456413show ga Cryo-electron tomography (cryoET) has become a powerful tool for direct visualization of 3D structures of native biological specimens at molecular resolution, but its application is limited to thin specimens (<300 nm). Recently, vitreous sectioning and cryo-FIB milling technologies were developed to physically reduce the specimen thickness; however, cryoET analysis of membrane protein complexes within native cell membranes remains a great challenge. Here, we use phage ?X174 lysis gene E to rapidly produce native, intact, bacterial cell membranes for high resolution cryoET. We characterized E gene-induced cell lysis using FIB/SEM and cryoEM and show that the bacteria cytoplasm was largely depleted through spot lesion, producing ghosts with the cell membranes intact. We further demonstrate the utility of E-gene-induced lysis for cryoET using the bacterial chemotaxis receptor signaling complex array. The described method should have a broad application for structural and functional studies of native, intact cell membranes and membrane protein complexes. äControlled Bacterial Lysis for Electron Tomography of Native Cell Memb(epmc).pdf DeepDyve Pubget Overpricing