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10.1016/j.brainres.2014.10.029 http://scihub22266oqcxt.onion/10.1016/j.brainres.2014.10.029 C4254496!4254496 !25451097     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25451097 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25451097 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Brain+Res 2014 ; 1593 (ä): 1-8 Nephropedia Template TP {{tp|p=25451097 |t=2014. p38 MAP kinase mediates transforming-growth factor-?1-induced upregulation of matrix metalloproteinase-9 but not -2 in human brain pericytes |pdf=|usr=}}{{25451097 }} gab.com Text p38 MAP kinase mediates transforming-growth factor- 1-induced upregulation of matrix metalloproteinase-9 but not -2 in human brain pericytes JO: Brain Res http://www.kidney.de/pget.php?&tw=1&pmid=25451097 #FOAvip Pericytes are vascular mural cells embedded within the basal lamina of blood micro-vessels. Within the neurovascular unit, pericytes play important roles in regulating neurovascular homeostasis by secreting soluble factors, such as matrix metalloproteinases (MMPs). However, little is known about the regulatory signaling pathways in brain pericytes. Here we show that transforming growth factor-?1 (TGF-?1) induces MMP-9 upregulation in pericytes via p38 mitogen-activated protein (MAP) kinase signaling. Cultured human brain vascular pericytes were used in this study. When the brain pericytes were treated with purified human TGF-?1 (0.1-10ng/mL for 24h), the levels of MMP-2 and MMP-9 in culture media were significantly increased in a concentration dependent manner as measured by gelatin zymography. WST assay confirmed that TGF-?1 did not affect cell survival of the brain pericytes. A TGF-?-receptor inhibitor SB431542 (0.5-5?M) decreased the TGF-?1-induced upregulation of MMP-2 and MMP-9. To assess the underlying intracellular mechanisms, we focused on p38 MAP kinase signaling, which is one of the major downstream kinases for TGF-?1. A well-validated p38 MAP kinase inhibitor SB203580 (0.5-5?M) cancelled the effect of TGF-?1 in upregulation of MMP-9 but not MMP-2. Western blotting confirmed that TGF-?1 treatment increased the level of p38 MAP kinase phosphorylation in pericytes, and again, the TGF-?-receptor inhibitor SB431542 (0.5-5?M) blocked the TGF-?1-induced phosphorylation of p38 MAP kinase. Both TGF-?1 and MMP-9 are major neurovascular mediators, and therefore, our current finding may suggest a novel mechanism for how pericytes regulate neurovascular homeostasis. Twit Text FOAVip p38 MAP kinase mediates transforming-growth factor- 1-induced upregulation of matrix metalloproteinase-9 but not -2 in human brain pericytes ????Brain Res http://www.kidney.de/pget.php?&tw=1&pmid=25451097 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25451097 #FOAvip English Wikipedia <ref>{{Cite pmid|25451097 }}</ref> p38 MAP kinase mediates transforming-growth factor-?1-induced upregulation of matrix metalloproteinase-9 but not -2 in human brain pericytes #MMPMID25451097 Takahashi Y ; Maki T ; Liang AC ; Itoh K ; Lok J ; Osumi N ; Arai K Brain Res 2014[Dec]; 1593 (ä): 1-8 PMID25451097 show ga Pericytes are vascular mural cells embedded within the basal lamina of blood micro-vessels. Within the neurovascular unit, pericytes play important roles in regulating neurovascular homeostasis by secreting soluble factors, such as matrix metalloproteinases (MMPs). However, little is known about the regulatory signaling pathways in brain pericytes. Here we show that transforming growth factor-?1 (TGF-?1) induces MMP-9 upregulation in pericytes via p38 mitogen-activated protein (MAP) kinase signaling. Cultured human brain vascular pericytes were used in this study. When the brain pericytes were treated with purified human TGF-?1 (0.1-10ng/mL for 24h), the levels of MMP-2 and MMP-9 in culture media were significantly increased in a concentration dependent manner as measured by gelatin zymography. WST assay confirmed that TGF-?1 did not affect cell survival of the brain pericytes. A TGF-?-receptor inhibitor SB431542 (0.5-5?M) decreased the TGF-?1-induced upregulation of MMP-2 and MMP-9. To assess the underlying intracellular mechanisms, we focused on p38 MAP kinase signaling, which is one of the major downstream kinases for TGF-?1. A well-validated p38 MAP kinase inhibitor SB203580 (0.5-5?M) cancelled the effect of TGF-?1 in upregulation of MMP-9 but not MMP-2. Western blotting confirmed that TGF-?1 treatment increased the level of p38 MAP kinase phosphorylation in pericytes, and again, the TGF-?-receptor inhibitor SB431542 (0.5-5?M) blocked the TGF-?1-induced phosphorylation of p38 MAP kinase. Both TGF-?1 and MMP-9 are major neurovascular mediators, and therefore, our current finding may suggest a novel mechanism for how pericytes regulate neurovascular homeostasis. |Benzamides/pharmacology [MESH] |Brain/drug effects/*metabolism [MESH] |Cell Survival/physiology [MESH] |Cells, Cultured [MESH] |Central Nervous System Agents/pharmacology [MESH] |Dioxoles/pharmacology [MESH] |Enzyme Inhibitors/pharmacology [MESH] |Humans [MESH] |Imidazoles/pharmacology [MESH] |MAP Kinase Signaling System [MESH] |Matrix Metalloproteinase 2/*metabolism [MESH] |Matrix Metalloproteinase 9/*metabolism [MESH] |Pericytes/drug effects/*metabolism [MESH] |Phosphorylation/drug effects [MESH] |Pyridines/pharmacology [MESH] |Receptors, Transforming Growth Factor beta/antagonists & inhibitors/metabolism [MESH] |Recombinant Proteins/metabolism [MESH] |Transforming Growth Factor beta1/*metabolism [MESH] |Up-Regulation/drug effects [MESH]p38 MAP kinase mediates transforming-growth factor-?1-induced upregula(epmc).pdf DeepDyve Pubget Overpricing