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10.1016/j.cell.2014.09.029 http://scihub22266oqcxt.onion/10.1016/j.cell.2014.09.029 C4253859!4253859!25307932     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2014 ; 159 (3): 647-61 Nephropedia Template TP {{tp|p=25307932|t=2014. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation |pdf=|usr=}}{{25307932}} gab.com Text Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=25307932 #FOAvip While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of transcript function lags behind. We present a robust technology enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts. We identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90?99% knockdown with minimal off-target effects, and activators (CRISPRa) to endogenous genes via endonuclease-deficient Cas9. Together they enable modulation of gene expression over a ~1000-fold range. Using these rules, we construct genome-scale CRISPRi and CRISPRa libraries, each of which we validate with two pooled screens. Growth-based screens identify essential genes, tumor suppressors and regulators of differentiation. Screens for sensitivity to a cholera-diphtheria toxin provide broad insights into the mechanisms of pathogen entry, retro-translocation and toxicity. Our results establish CRISPRi and CRISPRa as powerful tools that provide rich and complementary information for mapping complex pathways. Twit Text FOAVip Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=25307932 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25307932 #FOAvip English Wikipedia <ref>{{Cite pmid|25307932}}</ref> Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation #MMPMID25307932 Gilbert LA; Horlbeck MA; Adamson B; Villalta JE; Chen Y; Whitehead EH; Guimaraes C; Panning B; Ploegh HL; Bassik MC; Qi LS; Kampmann M; Weissman JS Cell 2014[Oct]; 159 (3): 647-61 PMID25307932show ga While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of transcript function lags behind. We present a robust technology enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts. We identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90?99% knockdown with minimal off-target effects, and activators (CRISPRa) to endogenous genes via endonuclease-deficient Cas9. Together they enable modulation of gene expression over a ~1000-fold range. Using these rules, we construct genome-scale CRISPRi and CRISPRa libraries, each of which we validate with two pooled screens. Growth-based screens identify essential genes, tumor suppressors and regulators of differentiation. Screens for sensitivity to a cholera-diphtheria toxin provide broad insights into the mechanisms of pathogen entry, retro-translocation and toxicity. Our results establish CRISPRi and CRISPRa as powerful tools that provide rich and complementary information for mapping complex pathways. äGenome-Scale CRISPR-Mediated Control of Gene Repression and Activation(epmc).pdf DeepDyve Pubget Overpricing