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2014 ; 123
(ä): 13-6
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Fucoidan stimulates cell division in the amebocyte-producing organ of the
schistosome-transmitting snail Biomphalaria glabrata
#MMPMID25233872
Sullivan JT
; Belloir JA
; Beltran RV
; Grivakis A
; Ransone KA
J Invertebr Pathol
2014[Nov]; 123
(ä): 13-6
PMID25233872
show ga
Adult Salvador (schistosome-resistant) strain Biomphalaria glabrata snails were
injected with 5 ?l of 10 mg/ml solutions of the sulfated polysaccharides ?
carageenan, dextran sulfate, fucoidan, and heparin, the nonsulfated
polysaccharide laminarin, and the monosaccharides L-fucose and L-galactose, and
mitotic activity in the amebocyte-producing organ (APO) was measured in
histological sections at 24 h post injection. Among the substances tested, only
fucoidan induced elevated mitotic activity. Desulfated fucoidan was not
mitogenic, indicating that sulfate groups are required for activity.
Schistosome-susceptible M-line snails possessed minimal or no hematopoietic
tissue in their APO, which did not respond to fucoidan. Immersion of juvenile
Salvador snails in 1 or 10 mg/ml solutions of fucoidan for 3 h did not elevate
mitotic activity at 24 h post immersion, suggesting that the external and
digestive tract epithelia of B. glabrata are impermeable to this molecule. These
results provide support for the hypothesis that fucosylated glycans on the
tegument and in excretory-secretory products of sporocysts of Schistosoma mansoni
are in part responsible for increased mitotic activity in the APO of B. glabrata
infected with this trematode or injected with its extracts.