Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25175010
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Anal+Biochem
2014 ; 466
(ä): 76-82
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Review of methods to assign the nuclear magnetic resonance peaks of reductively
methylated proteins
#MMPMID25175010
Roberson KJ
; Macnaughtan MA
Anal Biochem
2014[Dec]; 466
(ä): 76-82
PMID25175010
show ga
Reductive methylation of lysyl side-chain amines has been a successful tool in
the advancement of high-resolution structural biology. The utility of this method
has continuously gained ground as a protein chemical modification, first as a
tool to aid protein crystallization and later as a probe in protein nuclear
magnetic resonance (NMR) spectroscopy. As an isotope-labeling strategy for NMR
studies, reductive methylation has contributed to the study of protein-protein
interactions and global conformational changes. Although more detailed structural
studies using this labeling strategy are possible, the hurdle of assigning the
NMR peaks to the corresponding reductively methylated amine hinders its use. In
this review, we discuss and compare strategies used to assign the NMR peaks of
reductively methylated protein amines.