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10.1038/nmeth.3145

http://scihub22266oqcxt.onion/10.1038/nmeth.3145
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C4247985!4247985!25306545
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suck abstract from ncbi


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pmid25306545      Nat+Methods 2014 ; 11 (12): 1229-32
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  • A DNA-based molecular probe for optically reporting cellular traction forces #MMPMID25306545
  • Blakely BL; Dumelin CE; Trappmann B; McGregor LM; Choi CK; Anthony PC; Duesterberg VK; Baker BM; Block SM; Liu DR; Chen CS
  • Nat Methods 2014[Dec]; 11 (12): 1229-32 PMID25306545show ga
  • We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.
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