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2014 ; 14
(11
): 6407-13
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Azimuthal polarization filtering for accurate, precise, and robust
single-molecule localization microscopy
#MMPMID25272093
Lew MD
; Moerner WE
Nano Lett
2014[Nov]; 14
(11
): 6407-13
PMID25272093
show ga
Many single nanoemitters such as fluorescent molecules produce dipole radiation
that leads to systematic position errors in both particle tracking and
super-resolution microscopy. Via vectorial diffraction equations and simulations,
we show that imaging only azimuthally polarized light in the microscope naturally
avoids emission from the z-component of the transition dipole moment, resulting
in negligible localization errors for all emitter orientations and degrees of
objective lens misfocus. Furthermore, localization accuracy is maintained even in
the presence of aberrations resulting from imaging in mismatched media.