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10.1038/ki.2014.204 http://scihub22266oqcxt.onion/10.1038/ki.2014.204 C4245460!4245460!24940801     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Kidney+Int 2014 ; 86 (6): 1116-29 Nephropedia Template TP {{tp|p=24940801|t=2014. Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo |pdf=|usr=}}{{24940801}} gab.com Text Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo JO: Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=24940801 #FOAvip Identifying new biomarkers and therapeutic targets for podocytopathies such as focal segmental glomerulosclerosis (FSGS) requires a detailed analysis of transcriptional changes in podocytes over the course of disease. Here we used translating ribosome affinity purification (TRAP) to isolate and profile podocyte-specific mRNA in two different models of FSGS. Expressed eGFP-tagged ribosomal protein L10a in podocytes under the control of the Collagen-1?1 promoter enabled podocyte-specific mRNA isolation in a one-step process over the course of disease. This TRAP protocol robustly enriched known podocyte-specific mRNAs. We crossed col1?1-L10a mice with the actn4?/? and actn4+/K256E models of FSGS and analyzed podocyte transcriptional profiles at 2, 6 and 44 weeks of age. Two upregulated podocyte genes in murine FSGS (CXCL1 and DMPK) were found to be upregulated at the protein level in biopsies from patients with FSGS, validating this approach. There was no dilution of podocyte-specific transcripts during disease. These are the first podocyte-specific RNA expression datasets during aging and in two models of FSGS. This approach identified new podocyte proteins that are upregulated in FSGS and help define novel biomarkers and therapeutic targets for human glomerular disease. Twit Text FOAVip Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo ????Kidney Int http://www.kidney.de/pget.php?&tw=1&pmid=24940801 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24940801 #FOAvip English Wikipedia <ref>{{Cite pmid|24940801}}</ref> Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo #MMPMID24940801 Grgic I; Hofmeister AF; Genovese G; Bernhardy AJ; Sun H; Maarouf OH; Bijol V; Pollak MR; Humphreys BD Kidney Int 2014[Dec]; 86 (6): 1116-29 PMID24940801show ga Identifying new biomarkers and therapeutic targets for podocytopathies such as focal segmental glomerulosclerosis (FSGS) requires a detailed analysis of transcriptional changes in podocytes over the course of disease. Here we used translating ribosome affinity purification (TRAP) to isolate and profile podocyte-specific mRNA in two different models of FSGS. Expressed eGFP-tagged ribosomal protein L10a in podocytes under the control of the Collagen-1?1 promoter enabled podocyte-specific mRNA isolation in a one-step process over the course of disease. This TRAP protocol robustly enriched known podocyte-specific mRNAs. We crossed col1?1-L10a mice with the actn4?/? and actn4+/K256E models of FSGS and analyzed podocyte transcriptional profiles at 2, 6 and 44 weeks of age. Two upregulated podocyte genes in murine FSGS (CXCL1 and DMPK) were found to be upregulated at the protein level in biopsies from patients with FSGS, validating this approach. There was no dilution of podocyte-specific transcripts during disease. These are the first podocyte-specific RNA expression datasets during aging and in two models of FSGS. This approach identified new podocyte proteins that are upregulated in FSGS and help define novel biomarkers and therapeutic targets for human glomerular disease. äDiscovery of new glomerular disease-relevant genes by translational pr(epmc).pdf DeepDyve Pubget Overpricing