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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Am+Soc+Nephrol 2014 ; 25 (12): 2847-58 Nephropedia Template TP
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Lineage Tracing Reveals Distinctive Fates for Mesothelial Cells and Submesothelial Fibroblasts during Peritoneal Injury #MMPMID24854266
Chen YT; Chang YT; Pan SY; Chou YH; Chang FC; Yeh PY; Liu YH; Chiang WC; Chen YM; Wu KD; Tsai TJ; Duffield JS; Lin SL
J Am Soc Nephrol 2014[Dec]; 25 (12): 2847-58 PMID24854266show ga
Fibrosis of the peritoneal cavity remains a serious, life-threatening problem in the treatment of kidney failure with peritoneal dialysis. The mechanism of fibrosis remains unclear partly because the fibrogenic cells have not been identified with certainty. Recent studies have proposed mesothelial cells to be an important source of myofibroblasts through the epithelial?mesenchymal transition; however, confirmatory studies in vivo are lacking. Here, we show by inducible genetic fate mapping that type I collagen?producing submesothelial fibroblasts are specific progenitors of ?-smooth muscle actin?positive myofibroblasts that accumulate progressively in models of peritoneal fibrosis induced by sodium hypochlorite, hyperglycemic dialysis solutions, or TGF-?1. Similar genetic mapping of Wilms? tumor-1?positive mesothelial cells indicated that peritoneal membrane disruption is repaired and replaced by surviving mesothelial cells in peritoneal injury, and not by submesothelial fibroblasts. Although primary cultures of mesothelial cells or submesothelial fibroblasts each expressed ?-smooth muscle actin under the influence of TGF-?1, only submesothelial fibroblasts expressed ?-smooth muscle actin after induction of peritoneal fibrosis in mice. Furthermore, pharmacologic inhibition of the PDGF receptor, which is expressed by submesothelial fibroblasts but not mesothelial cells, attenuated the peritoneal fibrosis but not the remesothelialization induced by hypochlorite. Thus, our data identify distinctive fates for injured mesothelial cells and submesothelial fibroblasts during peritoneal injury and fibrosis.
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J+Am+Soc+Nephrol 2014 ; 25 (12): 2847-58
